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Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Chemical, Optical and Bioorthogonal Methods de David M. Chenoweth

Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Chemical, Optical and Bioorthogonal Methods: Methods in Enzymology, cartea 641

David M. Chenoweth
en Limba Engleză Hardback – 23 iul 2020
Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Chemical, Optical and Bioorthogonal Methods, Volume 641 in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this new release include caged cyclopropanes with improved tetrazine ligation kinetics, an analysis of metabolically labeled inositol phosphate messengers by NMR, cell-permeant caged inositol pyrophosphates for probing β-cells, imaging phospholipase D activity with clickable alcohols via transphosphatidylation, fluorescent biorthogonal labeling of class B GPCRs in live cells, near-infrared photoactivatable nitric oxide donors with integrated photoacoustic monitoring, and much more.


  • Provides the authority and expertise of leading contributors from an international board of authors
  • Presents the latest release in the Methods in Enzymology series
  • Includes the latest information on retinoid signaling pathways
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Specificații

ISBN-13: 9780128211564
ISBN-10: 0128211563
Pagini: 526
Dimensiuni: 152 x 229 mm
Greutate: 0.87 kg
Editura: ELSEVIER SCIENCE
Seria Methods in Enzymology

Public țintă

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists.

Cuprins

    1. Enzyme- or light-triggered cyclopropenes for bioorthogonal ligationTing Jiang and Scott T. Laughlin
    2. Analysis of metabolically labeled inositol phosphate messengers by NMRRobert Puschmann, Robert K. Harmel and Dorothea Fiedler
    3. Photo-releasable derivatives of inositol pyrophosphatesSebastian Hauke, Tamara Bittner, Henning J. Jessen and Carsten Schultz
    4. IMPACT: Imaging phospholipase D activity with clickable alcohols via transphosphatidylationTimothy W. Bumpus, Dongjun Liang and Jeremy M. Baskin
    5. Fluorescent bioorthogonal labeling of class B GPCRs in live cellsSrikanth Kumar Gangam and Qing Lin
    6. Near-infrared photoactivatable nitric oxide donors with photoacoustic readoutEffie Y. Zhou, Hailey J. Knox, Christopher J. Reinhardt, Gina Partipilo and Jefferson Chan
    7. H2S donors with optical responsesMichael D. Pluth, Yu Zhao and Matthew M. Cerda
    8. Visualization of oxidative stress-induced carbonylation in live mammalian cellsKamalika Mukherjee, Tak Ian Chio and Susan L. Bane
    9. Receptor-based fluorescent sensors constructed from ribonucleopeptideSkun Nakano, Hiroaki Konishi and Takashi Morii
    10. Conformationally restrained pentamethine cyanines and use in reductive single molecule localization microscopySiddharth S. Matikonda, Ralph Götz, Ryan McLaughlin, Markus Sauer and Martin J. Schnermann
    11. A near-infrared light-mediated cleavable linker strategy using the heptamethine cyanine chromophoreMichael P. Luciano, Saghar Nourian, Alexander P. Gorka, Roger R. Nani, Tadanobu Nagaya, Hisataka Kobayashi and Martin J. Schnermann
    12. Quantitative measurement of cytosolic penetration using the chloroalkane penetration assayKirsten Deprey and Joshua A. Kritzer
    13. Cell-penetrating and mitochondrion-targeting moleculesGeorge Appiah Kubi and Dehua Pei
    14. A photocaged DNA nanocapsule for delivery and manipulation in cellsYihong Feng, Takeshi Tohgasaki, Yasuyuki Shitomi, Hiroshi Sugiyama and Masayuki Endo
    15. A multicolor riboswitch-based platform for imaging of RNA in live mammalian cellsEsther Braselmann and Amy E. Palmer
    16. Enzymatic covalent labeling of RNA with RNA transglycosylation at guanosine (RNA-TAG)Kayla N. Busby and Neal K. Devaraj
    17. Assaying RNA solvent accessibility in living cells with LASERChao Feng and Robert C. Spitale
    18. Split T7 RNA polymerase biosensors to study multiprotein interaction dynamicsJeffrey A. Dewey and Bryan C. Dickinson
    19. Cross-linking cellular nucleic acids via a target-directing double click reagentMasayuki Tera and Nathan W. Luedtke
    20. Click chemistry-based amplification and detection of endogenous RNA and DNA molecules in situ using clampFISH probesSepideh Tavakoli, Yifang Liu, Jacob Potts and Sara H. Rouhanifard
    21. Quantification of protein delivery in live cells using fluorescence correlation spectroscopy
Susan L. Knox, Angela Steinauer, Garrett Alpha-Cobb, Adam Trexler, Elizabeth Rhoades and Alanna Schepartz