Chiral Separations (Methods in Molecular Biology) de Gerhard K.E. Scriba

Chiral Separations: Methods and Protocols: Methods in Molecular Biology, cartea 1985

Gerhard K.E. Scriba

This detailed book focuses on analytical separations by chromatographic and electrophoretic techniques, providing some overview along with numerous practically-oriented applications of the most important analytical techniques in chiral separation sciences. While some compounds may only be enantioseparated with one technique based on the physico-chemical properties, often the analyst can choose between two or more analytical techniques for a given analyte, which requires knowledge of the strengths and weaknesses of each technique in order to select the most appropriate method for the given problem. This collection binds that knowledge in one volume. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Chiral Separations: Methods and Protocols, Third Edition serves as a helpful guide for analytical chemists working on stereochemical problems in the fields of pharmacy, chemistry, biochemistry, food chemistry, molecular biology, forensics, environmental sciences, or cosmetics in academia, government, or industry.

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Cuprins

Recognition Mechanisms of Chiral Selectors: An Overview.- Enantioseparation by Thin Layer Chromatography.- Enantioseparations by Gas Chromatography Using Porous Organic Cages as Stationary Phase.- Chiral Metabolomics Using Triazine-Based Chiral Labeling Reagents by UPLC-ESI-MS/MS.- Chiral Mobile Phase Additives in HPLC Enantioseparations.- Polysaccharide-Based Chiral Stationary Phases for Enantioseparations by High Performance Liquid Chromatography: An Overview.- HPLC Enantioseparations with Polysaccharide-Based Chiral Stationary Phases in HILIC Conditions.- Functional Cyclodextrin-Clicked Chiral Stationary Phases for Versatile Enantioseparations by HPLC.- HPLC Enantioseparation on Cyclodextrin-Based Chiral Stationary Phases.- Hybrid Organic-Inorganic Materials Containing a Nanocellulose Derivative as Chiral Selector.- Cyclofructans as Chiral Selectors: An Overview.- High-Performance Liquid Chromatography Enantioseparations Using Macrocyclic Glycopeptide-Based Chiral Stationary Phases: An Overview.- Application of Sub-2 Micron Particles Silica Hydride Derivatized with Vancomycin for Chiral Separations by Nano-Liquid Chromatography.- Cinchona Alkaloid-Based Zwitterionic Chiral Stationary Phases Applied for Liquid Chromatographic Enantiomer Separations: An Overview.- Enantioseparations by High Performance Liquid Chromatography Based on Chiral Ligand-Exchange.- Applications of Chiral Supercritical Fluid Chromatography.- Chiral Separations by Countercurrent Chromatography.- Cyclodextrins as Chiral Selectors in Capillary Electrophoresis Enantioseparations.- Application of Dual Cyclodextrin-Systems in Capillary Electrophoresis Enantioseparations.- Enantioseparation by Capillary Electrophoresis Using Cyclodextrins in an Amino Acid-Based Ionic Liquid Running Buffer.- Enantioseparations in Nonaqueous Capillary Electrophoresis Using Charged Cyclodextrins.- Chiral Separation by NACE Using Polyol Derivative–Boric Acid Complexes.- Chiral Capillary Electrophoresis-Mass Spectrometry.- Enantioseparation of Selected Imidazole Drugs Using Dual Cyclodextrin-Modified Micellar Electrokinetic Chromatography.- Carbohydrate-Based Polymeric Surfactants for Chiral Micellar Electrokinetic Chromatography (CMEKC) Coupled to Mass Spectrometry.- Application of a (18-Crown-6)-2,3,11,12-Tetracarboxylic Acid-Based Chiral Stationary Phase in Capillary Electrochromatography.- Experimental Design Methodologies for the Optimization of Chiral Separations: An Overview.

Recenzii

“The book is aimed at investigators in any discipline working in the field of chirality. It will be of special interest to anyone who has a need to separate chiral molecules. … Detailed protocols are provided for the systems covered in the book that will make it easier for investigators wishing to replicate one of these procedures.” (Thomas J. Wenzel, Analytical and Bioanalytical Chemistry, Vol. 411, 2019)

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