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Clinical Applications of Mass Spectrometry in Biomolecular Analysis: Methods and Protocols de Uttam Garg

Clinical Applications of Mass Spectrometry in Biomolecular Analysis: Methods and Protocols: Methods in Molecular Biology, cartea 2546

Editat de Uttam Garg
en Limba Engleză Paperback – 22 sep 2023
This updated volume provides stepwise instructions for the analysis of numerous clinically important analytes by mass spectrometry. Mass spectrometry offers clinical laboratory scientists a number of advantages including increased sensitivity and specificity, multiple component analysis, and limited need for specialized reagents. These techniques are essential in laboratory fields including endocrinology, biochemical genetics, drug analysis, proteomics, and pathogen identification. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Cutting-edge and practical, Clinical Applications of Mass Spectrometry in Biomolecular Analysis: Methods and Protocols, Second Edition is an ideal resource for clinical laboratory scientists who are already using or thinking of bringing mass spectrometry to their laboratories.
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Specificații

ISBN-13: 9781071625675
ISBN-10: 1071625675
Pagini: 547
Ilustrații: XVI, 547 p. 88 illus., 55 illus. in color.
Dimensiuni: 178 x 254 mm
Greutate: 0.97 kg
Ediția:2nd ed. 2022
Editura: Springer Us
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:New York, NY, United States

Cuprins

Introduction to Mass Spectrometry for Bimolecular Analysis in a Clinical Laboratory.- System Performance Monitoring in Clinical Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS).- Tandem Mass Spectrometry for the Analysis of Plasma/Serum Acylcarnitines for the Diagnosis of Certain Organic Acidurias and Fatty Acid Oxidation Disorders.- Quantification of Plasma S-Adenosylmethionine and S-Adenosylhomocysteine Using Liquid Chromatography Electrospray Tandem Mass Spectrometry.- Quantitation of Aldosterone in Serum or Plasma Using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS).- Comprehensive Determination of Amino Acids for Diagnosis of Inborn Errors of Metabolism.- Quantification of Branched-Chain Amino Acids in Plasma by High-Performance Liquid Chromatography-Tandem Mass Spectrometry (LC- MS/MS).- Quantitation of Butyrylcarnitine, Isobutyrylcarnitine, and Glutarylcarnitine in Urine Using Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry (UPLC-MS/MS).- Quantification of Free and Total Carnitine in Serum Using Liquid Chromatography Tandem Mass Spectrometry.- Sensitive and Robust LC-MS/MS Analysis of Salivary Cortisol in Negative Mode.- Measurement of Urine Free Cortisol and Cortisone by LC-MS/MS.- Laboratory Diagnosis of Cerebral Creatine Deficiency Syndromes by Determining Creatine and Guanidinoacetate in Plasma and Urine.- Quantitation of Estradiol and Testosterone in Serum Using LC-MS/MS.- Quantitation of Fatty Acids in Serum/Plasma and Red Blood Cells by Gas Chromatography-Negative Chemical Ionization-Mass Spectrometry.- Quantitation of γ-Aminobutyric Acid in Cerebrospinal Fluid Using Liquid Chromatography Electrospray Tandem Mass Spectrometry.- A Simple, Fast, and Reliable LC-MS/MS Method for the Measurement of Homovanillic Acid and Vanillylmandelic Acid in Urine Specimens.- Quantitation of Neuroblastoma Markers Homovanillic Acid (HVA) and Vanillylmandelic Acid (VMA) in Urine by Gas Chromatography-Mass Spectrometry (GC/MS).- Quantification of 5-Hydroxyindoleacetic Acid in Urine by Ultra Performance Liquid Chromatography Tandem Mass Spectrometry.- Quantitation of IgG Subclasses in Serum Using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS).- Quantification of Insulin Analogs by Liquid Chromatography-High Resolution Mass Spectrometry.- Differentiation of Common IGF-1 Variants Using HRAM-MS COM Determination with Follow-Up MS/MS Verification.- Monitoring and Identifying Insulin-Like Growth Factor 1 Variants by Liquid Chromatography-High Resolution Mass Spectrometry in a Clinical Laboratory.- Quantitation of Lactate in Cerebrospinal Fluid Using Liquid Chromatography-Electrospray Tandem Mass Spectrometry.- Multiplex Lysosomal Enzyme Activity Assay on Dried Blood Spots Using Tandem Mass Spectrometry.- Plasma Lysosphingolipid Biomarker Measurement by Liquid Chromatography-Tandem Mass Spectrometry.- Detection of 13C-Mannitol and Other Saccharides Using Tandem Mass Spectrometry for Evaluation ofIntestinal Permeability, or Leaky Gut.- LC-MS/MS Method for High Throughput Analysis of Methylmalonic Acid in Serum, Plasma, and Urine: Method for Analyzing Isomers without Chromatographic Separation.- Quantitation of 5-Methyltetrahydrofolate in Cerebrospinal Fluid Using Liquid Chromatography Electrospray Tandem Mass Spectrometry.- Screening of Organic Acidurias by Gas Chromatography Mass Spectrometry (GC-MS).- Identification of Urine Organic Acids for the Detection of Inborn Errors of Metabolism Using Urease and Gas Chromatography-Mass Spectrometry (GC/MS).- Quantitative Organic Acids in Urine by Two-Dimensional Gas Chromatography-Time-of-Flight Mass Spectrometry (GCxGC-TOFMS).- Quantification of Parathyroid Hormone and Its Fragments in Serum by Liquid Chromatography-High Resolution Mass Spectrometry.- High Sensitivity Measurement of Parathyroid Hormone Related Protein (PTHrP) in Plasma by LC-MS/MS.- Quantitation of Phenylalanine in Dried Blood Spot using Liquid Chromatography TandemMass Spectrometry for Monitoring of Patients with Phenylketonuria (PKU).- An Optimized Procedure for Proteomic Analysis of Extracellular Vesicles Using In-Stage Tip Digestion and DIA LC-MS/MS: Application to Liquid Biopsy in Cancer.- High-Throughput Plasma Proteomic Profiling.- Quantitation of Purine in Urine by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry.- Quantitation of Pyrimidine in Urine by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry.- Quantitation of Renin Activity in Plasma Using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS).- Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) Method for the Quantification Steroids Androstenedione, Dehydroepiandrosterone, 11-Deoxycortisol, 17-Hydroxyprogesterone, and Testosterone.- A User-Friendly Sample Preparation Alternative for Manual and Automated LC-MS/MS Quantification of Testosterone.- Quantitation of Thyroglobulin in Serum Using SISCAPA and Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS).- Quantitation of Free Thyroxine by Equilibrium Dialysis and Liquid Chromatography-Tandem Mass Spectrometry.- Quantification of Tryptophan, Indole, and Indoxyl Sulfate in Urine Using Liquid Chromatography-Tandem Mass Spectrometry.- Very Long-Chain Fatty Acids Quantification by Gas-Chromatography Mass Spectrometry.- Quantification of Very Long-Chain and Branched-Chain Fatty Acids in Plasma by Liquid Chromatography-Tandem Mass Spectrometry.- High Throughput Analysis of 25-OH-Vitamin D2 and D3 Using Multi-Channel Liquid Chromatography-Tandem Mass Spectrometry.- Quantification of 25-Hydroxyvitamin D2 and D3 Using Liquid Chromatography-Tandem Mass Spectrometry.

Textul de pe ultima copertă

This updated volume provides stepwise instructions for the analysis of numerous clinically important analytes by mass spectrometry. Mass spectrometry offers clinical laboratory scientists a number of advantages including increased sensitivity and specificity, multiple component analysis, and limited need for specialized reagents. These techniques are essential in laboratory fields including endocrinology, biochemical genetics, drug analysis, proteomics, and pathogen identification. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Cutting-edge and practical, Clinical Applications of Mass Spectrometry in Biomolecular Analysis: Methods and Protocols, Second Edition is an ideal resource for clinical laboratory scientists who are already using or thinking of bringing mass spectrometry to their laboratories.

Caracteristici

Includes cutting-edge techniques Provides step-by-step detail essential for reproducible results Contains key implementation advice from the experts

Recenzii

“This is a compendium of mass spectrometry protocols for clinically important analytes. … This book is intended for anyone interested in clinical applications of mass spectrometry. It would be of interest to basic scientists, clinical laboratory practitioners (e.g., scientists, physicians, etc.), manufacturers, and healthcare providers interested in applications of mass spectrometry. … This is your book if you are contemplating implementing mass spectrometry in your laboratory for any of these clinically important analytes.” (Valerie L. Ng, Doody's Book Reviews, March, 2016)