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Clinical Applications of Mass Spectrometry: Methods and Protocols de Uttam Garg

Clinical Applications of Mass Spectrometry: Methods and Protocols: Methods in Molecular Biology, cartea 603

Editat de Uttam Garg, Catherine A. Hammett-Stabler
en Limba Engleză Hardback – 14 dec 2009
As mass spectrometric methods now offer a level of specificity and sensitivity unrealized by spectrophotometric- and immunoassay-based methods, mass spectrometry has entered the clinical laboratory where it is being used for a wide range of applications. In Clinical Applications of Mass Spectrometry: Methods and Protocols, expert researchers provide detailed step-by-step procedures for the analysis of number of analytes of clinical importance. This versatile and expansive volume covers mass spectrometry methods for analytes including a variety of drugs, hormones, and metabolic compounds spanning the disciplines of toxicology, therapeutic drug monitoring, endocrinology, and pediatric metabolism. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to the analytes, lists of the necessary materials and reagents, readily reproducible analytical protocols, and detailed notes on troubleshooting and avoiding known pitfalls.
Comprehensive and dependable, Clinical Applications of Mass Spectrometry: Methods and Protocols offers its readers a wide array of valuable methods for experienced mass spectrometric labs that are looking to introduce new analyses as well as for those laboratories currently considering the addition of this resourceful and vital technology.
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Specificații

ISBN-13: 9781607614586
ISBN-10: 1607614588
Pagini: 556
Ilustrații: XVI, 538 p.
Dimensiuni: 178 x 254 x 45 mm
Greutate: 1.18 kg
Ediția:2010
Editura: Humana Press Inc.
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:Totowa, NJ, United States

Public țintă

Professional/practitioner

Cuprins

The Evolution of Mass Spectrometry in the Clinical Laboratory.- Measurement of Plasma/Serum Acylcarnitines Using Tandem Mass Spectrometry.- Comprehensive Determination of Amino Acids for Diagnosis of Inborn Errors of Metabolism.- Identification and Quantitation of Amphetamine, Methamphetamine, MDMA, Pseudoephedrine, and Ephedrine in Blood, Plasma, and Serum Using Gas Chromatography-Mass Spectrometry (GC/MS).- Quantification of Antidepressants Using Gas Chromatography-Mass Spectrometry.- Quantitation of Argatroban in Plasma using Liquid Chromatography Electrospray Tandem Mass Spectrometry (UPLC-ESI-MS/MS).- Quantitation of Amobarbital, Butalbital, Pentobarbital, Phenobarbital, and Secobarbital in Urine, Serum, and Plasma Using Gas Chromatography-Mass Spectrometry (GC-MS).- Quantitation of Benzodiazepines in Blood and Urine Using Gas Chromatography-Mass Spectrometry (GC-MS).- LC-MS/MS Analysis of 13 Benzodiazepines and Metabolites in Urine, Serum, Plasma, and Meconium.- Simultaneous Determination and Quantification of 12 Benzodiazepines in Serum or Whole Blood Using UPLC/MS/MS.- Determination of Benzoic Acid in Serum or Plasma by Gas Chromatography-Mass Spectrometry (GC/MS).- Quantification of Busulfan in Plasma Uing Liquid Chromatography Electrospray Tandem Mass Spectrometry (HPLC-ESI-MS/MS).- Quantitation of Total 11-Nor-9-Carboxy-Delta 9-Tetrahydrocannabinol in Urine and Blood Using Gas Chromatography-Mass Spectrometry (GC-MS).- Quantitation of Cocaine, Benzoylecgonine, Ecgonine Methyl Ester, and Cocaethylene in Urine and Blood Using Gas Chromatography-Mass Spectrometry (GC-MS).- Identification and Quantitation of Cocaine, Benzoylecgonine, and Cocaethylene in Blood, Serum, and Plasma Using Ultra-Performance Liquid Chromatography Coupled to Tandem Mass Spectrometry (UPLC-MS/MS).- Detection and Quantification of Cocaine and Benzoylecgonine in Meconium Using Solid Phase Extraction and UPLC/MS/MS.- Diagnosis of Creatine Metabolism Disorders by Determining Creatine and Guanidinoacetate in Plasma and Urine.- Broad Spectrum Drug Screening Using Electron-Ionization Gas Chromatography-Mass Spectrometry (EI-GCMS).- Broad Spectrum Drug Screening Using Liquid Chromatography-Hybrid Triple Quadrupole Linear Ion Trap Mass Spectrometry.- High Sensitivity Measurement of Estrone and Estradiol in Serum and Plasma Using LC-MS/MS.- 3-Hydroxy-Fatty Acid Analysis by Gas Chromatography-Mass Spectrometry.- Quantitation of Fentanyl in Blood and Urine Using Gas Chromatography-Mass Spectrometry (GC-MS).- Determination of Total Homocysteine in Plasma Using Liquid Chromatography Coupled to Tandem Mass Spectrometry (LC/MS/MS).- Quantitation of Homovanillic Acid (HVA) and Vanillylmandelic Acid (VMA) in Urine Using Gas Chromatography-Mass Spectrometry (GC/MS).- Quantitation of 17-OH-Progesterone (OHPG) for Diagnosis of Congenital Adrenal Hyperplasia (CAH).- Determination of Hydroxyurea in Serum or Plasma Using Gas Chromatography-Mass Spectrometry (GC/MS).- Quantitation of Ibuprofen in Blood Using Gas Chromatography-Mass Spectrometry (GC-MS).- Quantitation of Indomethacin in Serum and Plasma Using Gas Chromatography-Mass Spectrometry (GC-MS).- Quantitation of Iothalamate in Urine and Plasma Using Liquid Chromatography Electrospray Tandem Mass Spectrometry (HPLC-ESI-MS/MS).- Identification and Quantitation of Ketamine in Biological Matrices Using Gas Chromatography-Mass Spectrometry (GC-MS).- Measurement of Filter Paper Bloodspot Lead by Inductively Coupled Plasma-Mass Spectrometry (ICP-MS).- Multiplex Lysosomal Enzyme Activity Assay on Dried Blood Spots Using Tandem Mass Spectrometry.- Gas Chromatography-Mass Spectrometry Method for the Determination of Methadone and 2-Ethylidene-1,5-Dimethyl-3,3-Diphenylpyrrolidine (EDDP).- Liquid Chromatography-Tandem Mass Spectrometry (LC-MSMS) Method for Monitoring Methotrexate in the Setting of Carboxypeptidase-G2 Therapy.- Quantitation of Methylmalonic Acid in Serum or Plasma Using Isotope Dilution-Selected Ion Gas Chromatography-Mass Spectrometry.- Quantitation of Methylmalonic Acid in Plasma Using Liquid Chromatography–Tandem Mass Spectrometry.- Monitoring of Mycophenolate Acid in Serum or Plasma Using LC Tandem Mass Spectrometry.- Quantitation of Nicotine, Its Metabolites, and Other Related Alkaloids in Urine, Serum, and Plasma Using LC-MS/MS.- Quantitation of Opioids in Blood and Urine Using Gas Chromatography-Mass Spectrometry (GC-MS).- Quantitation of Morphine, Codeine, Hydrocodone, Hydromorphone, Oxycodone, Oxymorphone, and 6-Monoacetylmorphine (6-MAM) in Urine, Blood, Serum, or Plasma Using Liquid Chromatography with Tandem Mass Spectrometry Detection.- Urine Organic Acid Analysis for Inherited Metabolic Disease Using Gas Chromatography-Mass Spectrometry.- Identification of Urine Organic Acids for the Detection of Inborn Errors of Metabolism Using Urease and Gas Chromatography-Mass Spectrometry (GC/MS).- Quantitation of Orotic Acid in Urine Using Isotope Dilution-Selected Ion Gas Chromatography-Mass Spectrometry.- Quantitation of Oxycodone in Blood and Urine Using Gas Chromatography-Mass Spectrometry (GC-MS).- Quantitation of Phencyclidine (PCP) in Urine and Blood Using Gas Chromatography-Mass Spectrometry (GC-MS).- Quantitation of Sirolimus Using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS-MS).- Quantitation of Tacrolimus in Whole Blood Using High Performance Liquid Chromatography-Tandem Mass Spectrometry (HPLC/MS/MS).- Analysis of Testosterone in Serum Using Mass Spectrometry.- Determination of Tetrahydrozoline in Urine and Blood Using Gas Chromatography-Mass Spectrometry (GC/MS).- Quantitation of 25-OH-Vitamin D (25OHD) Using Liquid Tandem Mass Spectrometry (LC-MS/MS).- Identification and Quantitation of Zolpidem in Biological Matrices Using Gas Chromatography-Mass Spectrometry (GC-MS).- Identification and Quantitation of Zopiclone in Biological Matrices Using Gas Chromatography-Mass Spectrometry (GC-MS).

Recenzii

From the reviews:
“This book provides a collection of over 50 applications of GC-MS, LC-MS, and ICP-MS for screening, identification, and quantification of clinically relevant analytes in body samples. … It can be recommended … to critical practitioners in laboratory medicine.” (Hans H. Maurer, Analytical and Bioanalytical Chemistry, Vol. 399, 2011)
“Clinical Applications of Mass Spectrometry Methods and Protocols is a compilation of ready-to-use protocols for the determination of clinically relevant analytes in various biological matrices. … the book is a straightforward compilation of diverse protocols written in a very user-friendly format. It is especially valuable for labs that have just started implementing mass spectrometry or for those planning to do so in the near future. For labs with more experience in mass spectrometry, the protocols can save many hours of precious method development time.” (Robert Jansen, Journal of the American Society for Mass Spectrometry, Vol. 22, 2011)

Textul de pe ultima copertă

As mass spectrometric methods now offer a level of specificity and sensitivity unrealized by spectrophotometric- and immunoassay-based methods, mass spectrometry has entered the clinical laboratory where it is being used for a wide range of applications. In Clinical Applications of Mass Spectrometry: Methods and Protocols, expert researchers provide detailed step-by-step procedures for the analysis of number of analytes of clinical importance. This versatile and expansive volume covers mass spectrometry methods for analytes including a variety of drugs, hormones, and metabolic compounds spanning the disciplines of toxicology, therapeutic drug monitoring, endocrinology, and pediatric metabolism. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to the analytes, lists of the necessary materials and reagents, readily reproducible analytical protocols, and detailed notes on troubleshooting and avoiding known pitfalls.
Comprehensive and dependable, Clinical Applications of Mass Spectrometry: Methods and Protocols offers its readers a wide array of valuable methods for experienced mass spectrometric labs that are looking to introduce new analyses as well as for those laboratories currently considering the addition of this resourceful and vital technology.

Caracteristici

Covers a range of gas chromatography- and liquid chromatography-based mass spectrometry techniques and systems Includes multiple analytical options presented for many analytes Serves as a comprehensive resource of mass spectrometry procedures for the clinical laboratory whether new to MS or experienced Contains well-organized procedures that will greatly facilitate assay implementations Provides a resource for materials and hints for troubleshooting problems Covers a wide range of analytes including hormones, drugs, and metabolites Includes supplementary material: sn.pub/extras