Cantitate/Preț
Produs
Total produse
Vezi coșul
Heterologous Expression of Membrane Proteins: Methods and Protocols de Isabelle Mus-Veteau

Heterologous Expression of Membrane Proteins: Methods and Protocols: Methods in Molecular Biology, cartea 1432

Editat de Isabelle Mus-Veteau
en Limba Engleză Paperback – 30 mai 2018
This detailed volume encompasses chapters from leadingexperts in the area of membrane proteins who describe step-by-step protocols developed these last few years to improve the functionalproduction and stabilization of recombinant integral membrane proteins (IMPs). Membraneproteins play a key role in numerous pathologies such as cancer, cysticfibrosis, epilepsy, hyperinsulinism, and Alzheimer’s disease, yet studies onthese and other disorders are hampered by a lack of information about theproteins involved. This book sets out to aid researchers in rectifying thissituation. Written for the highly successful Methods in Molecular Biology series, chapters include introductionsto their respective topics, lists of the necessary materials and reagents,readily reproducible laboratory protocols, and tips on troubleshooting andavoiding known pitfalls.
Authoritative and up-to-date, HeterologousExpression of Membrane Proteins: Methods and Protocols, Second Editionserves as an ideal guide for scientists attempting to delve deeper into the myriadunique IMP structures.

Citește tot Restrânge

Toate formatele și edițiile

Toate formatele și edițiile Preț Express
Paperback (3) 64643 lei  6-8 săpt.
  Springer – 30 mai 2018 64643 lei  6-8 săpt.
  Humana Press Inc. – 23 aug 2016 69977 lei  6-8 săpt.
  Springer Us – 2 iul 2023 95919 lei  6-8 săpt.
Hardback (3) 65412 lei  6-8 săpt.
  Springer – 3 aug 2016 65412 lei  6-8 săpt.
  Humana Press Inc. – 18 dec 2009 95365 lei  6-8 săpt.
  Springer Us – iul 2022 140241 lei  6-8 săpt.

Din seria Methods in Molecular Biology

  • 9% Nucleic Acid and Peptide Aptamers: Methods and Protocols
    Preț: 79159 lei
  • 23% Pheromone Signaling: Methods and Protocols
    Preț: 59856 lei
  • Engineering and Analyzing Multicellular Systems: Methods and Protocols
    Preț: 49679 lei
  • 20% Clinical Bioinformatics
    Preț: 88295 lei
  • Mitochondrial Regulation: Methods and Protocols
    Preț: 25204 lei
  • 5% Multiple Sclerosis: Methods and Protocols
    Preț: 72961 lei
  • 5% Bacterial Persistence: Methods and Protocols
    Preț: 73143 lei
  • 5% DNA-Protein Interactions: Principles and Protocols
    Preț: 74130 lei
  • 5% Methods in Mouse Atherosclerosis
    Preț: 74716 lei
  • 15% Cell Cycle Oscillators: Methods and Protocols
    Preț: 66345 lei
  • 18% TGF-β Signaling: Methods and Protocols
    Preț: 102534 lei
  • 5% Whole Genome Amplification: Methods and Protocols
    Preț: 73457 lei
  • 18% Patient-Specific Induced Pluripotent Stem Cell Models: Generation and Characterization
    Preț: 91420 lei
  • 15% Phospho-Proteomics: Methods and Protocols
    Preț: 66461 lei
  • 15% Candida Species: Methods and Protocols
    Preț: 65412 lei
  • 18% Induced Pluripotent Stem (iPS) Cells: Methods and Protocols
    Preț: 141474 lei
  • 5% Post-Transcriptional Gene Regulation
    Preț: 74260 lei
  • 20% In Vitro Embryogenesis in Higher Plants
    Preț: 82163 lei
  • 18% Yeast Functional Genomics: Methods and Protocols
    Preț: 97230 lei
  • 15% Statistical Analysis in Proteomics
    Preț: 66049 lei
  • 5% SiRNA Delivery Methods: Methods and Protocols
    Preț: 73841 lei
  • 18% Estrogen Receptors: Methods and Protocols
    Preț: 98492 lei
  • 5% Suppression and Regulation of Immune Responses: Methods and Protocols, Volume II
    Preț: 73329 lei
  • Plant Bioinformatics: Methods and Protocols
    Preț: 39258 lei
  • 5% Breast Cancer: Methods and Protocols
    Preț: 74626 lei
  • 18% Endocannabinoid Signaling: Methods and Protocols
    Preț: 96266 lei
  • 23% Zebrafish: Methods and Protocols
    Preț: 86021 lei
  • 15% Activity-Based Proteomics: Methods and Protocols
    Preț: 65264 lei
  • 5% Drug Target miRNA: Methods and Protocols
    Preț: 105550 lei
  • 23% Computational Protein Design
    Preț: 88385 lei
  • SH2 Domains: Methods and Protocols
    Preț: 79216 lei
  • Cholesterol Homeostasis: Methods and Protocols
    Preț: 42362 lei
  • 5% The Immune Synapse: Methods and Protocols
    Preț: 42591 lei
  • Organ Regeneration: 3D Stem Cell Culture & Manipulation
    Preț: 59220 lei
  • 5% Innate Antiviral Immunity: Methods and Protocols
    Preț: 34562 lei
  • 19% Protein Engineering: Methods and Protocols
    Preț: 49188 lei
  • 5% Innate Immune Activation: Methods and Protocols
    Preț: 103884 lei
  • 5% Investigations of Early Nutrition Effects on Long-Term Health: Methods and Applications
    Preț: 52415 lei
  • 18% Planarian Regeneration: Methods and Protocols
    Preț: 212234 lei
  • 5% Epitope Mapping Protocols
    Preț: 129923 lei
  • Tissue Proteomics: Methods and Protocols
    Preț: 78993 lei
  • 5% Genetic Epidemiology: Methods and Protocols
    Preț: 133910 lei
  • 18% Plant Chemical Genomics: Methods and Protocols
    Preț: 139026 lei
  • 5% Lung Innate Immunity and Inflammation: Methods and Protocols
    Preț: 75266 lei
  • 5% Rational Drug Design: Methods and Protocols
    Preț: 37489 lei
  • 18% Microbiome Analysis: Methods and Protocols
    Preț: 139563 lei
  • 18% Computational Methods in Protein Evolution
    Preț: 112965 lei
  • 18% Electrophoretic Separation of Proteins: Methods and Protocols
    Preț: 140826 lei
  • 18% Microbial Metabolomics: Methods and Protocols
    Preț: 112492 lei
  • 18% SNAREs: Methods and Protocols
    Preț: 96627 lei

Preț: 64643 lei

Preț vechi: 76050 lei
-15% Nou

Puncte Express: 970
Preț estimativ în valută:
12372€ • 12882$ • 10455£

Carte tipărită la comandă

Livrare economică 07-21 martie

 Adaugă în coș

Wish list
Gift list
Am citit!
Adaugă în listă
Preluare comenzi: 021 569.72.76

Specificații

ISBN-13: 9781493981021
ISBN-10: 1493981021
Ilustrații: XII, 322 p. 66 illus., 39 illus. in color.
Dimensiuni: 178 x 254 mm
Greutate: 0.59 kg
Ediția:Softcover reprint of the original 2nd ed. 2016
Editura: Springer
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:New York, NY, United States

Cuprins

Cell-Free Production of Membrane Proteins in Escherichia coli Lysates for Functionaland Structural Studies.- Membrane Protein Production in the Yeast, S. cerevisiae.- Membrane ProteinsProduction in Escherichia coli:Protocols and Rules.- Codon Optimizing for Increased Membrane ProteinProduction: A Minimalist Approach.- Generation of Tetracycline-InducibleMammalian Cell Lines by Flow Cytometry for Improved Overproduction of MembraneProteins.- Membrane Protein Production in Lactococcus lactis for FunctionalStudies.- Expression ofViral Envelope Glycoproteins in Drosophila melanogaster S2 Cells.- Leishmania tarentolæ as a Promising Toolfor Expressing Polytopic and Multi-Transmembrane Spans Eukaryotic MembraneProteins: The Case of the ABC Pump ABCG6.- Overexpression, MembranePreparation, and Purification of a Typical Multidrug ABC Transporter BmrA.- Expression ofEukaryotic Membrane Proteins in Pichiapastoris.- Integral Membrane Protein Expression in Saccharomyces cerevisiae.- High-Throughput Baculovirus ExpressionSystem for Membrane Protein Production.- Small-Scale Screening to Large-ScaleOver-Expression of Human Membrane Proteins for Structural Studies.- Purificationof Human and Mammalian Membrane Proteins Expressed in Xenopus laevis Frog Oocytes for Structural Studies.- MembraneProtein Solubilization and Composition of Protein Detergent Complexes.- Detergent-FreeMembrane Protein Purification.- Conformational Dynamics and Interactions ofMembrane Proteins by Hydrogen/Deuterium Mass Spectrometry.- Lessons from an a-Helical Membrane Enzyme: Expression,Purification, and Detergent Optimization for Biophysical and StructuralCharacterization.- Method to Screen Multidrug Transport Inhibitors Using YeastOver-Expressing a Human MDR Transporter.

Textul de pe ultima copertă

This detailed volume encompasses chapters from leading experts in the area of membrane proteins who describe step-by-step protocols developed these last few years to improve the functional production and stabilization of recombinant integral membrane proteins (IMPs). Membrane proteins play a key role in numerous pathologies such as cancer, cystic fibrosis, epilepsy, hyperinsulinism, and Alzheimer’s disease, yet studies on these and other disorders are hampered by a lack of information about the proteins involved. This book sets out to aid researchers in rectifying this situation. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, Heterologous Expression of Membrane Proteins: Methods and Protocols, Second Edition serves as an ideal guide for scientists attempting to delve deeper into the myriad unique IMP structures.

Caracteristici

Includes cutting-edge methods and protocols for the study of recombinant integral membrane Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Recenzii

From the reviews:
“Well-illustrated book provides an overview of different heterologous expression systems available to produce membrane proteins for structural analysis to facilitate understanding of how these proteins function and how their functions can be modified by drug treatment. … Written for students and experienced researchers, the book also will be useful for clinicians interested in the care of patients with cancer … . provides valuable information on membrane proteins for novice and experienced researchers. Clinicians will find it extremely useful for developing methods and protocols for research.” (Omer Iqbal, Doody’s Review Service, July, 2010)