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High-Throughput Screening Assays in Toxicology de Hao Zhu

High-Throughput Screening Assays in Toxicology: Methods in Molecular Biology, cartea 1473

Editat de Hao Zhu, Menghang Xia
en Limba Engleză Hardback – 13 aug 2016
This book focuses on recently developed High Throughput Screening (HTS) assay protocols, many involved in the ToxCast and/or Tox21 initiatives, and the relevant HTS data analysis techniques. Divided into three sections, in vitro assays, in vivo assays, and computational techniques to analyze HTS data are all examined. Written for the highly successful Methods in Molecular Biology series, most chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, High Throughput Screening Assays in Toxicology serves as a valuable reference resource for translating new HTS techniques into standardized chemical toxicity assessment tools in order to advance modern toxicology research to a new era where HTS techniques can partially replace the prevailing animal models.
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Specificații

ISBN-13: 9781493963447
ISBN-10: 1493963449
Pagini: 172
Ilustrații: XII, 175 p. 50 illus., 32 illus. in color.
Dimensiuni: 178 x 254 x 13 mm
Greutate: 0.54 kg
Ediția:1st ed. 2016
Editura: Springer
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:New York, NY, United States

Cuprins

Monitoring Ligand-Activated Protein-Protein Interactions Using Bioluminescent Resonance Energy Transfer (BRET) Assay.- Mitochondrial Membrane Potential Assay.- Using β-Lactamase and NanoLuc Luciferase Reporter Gene Assays to Identify Inhibitors of the HIF-1 Signaling Pathway.- Quantitative High-Throughput Luciferase Screening in Identifying CAR Modulators.- Transactivation and Coactivator Recruitment Assays for Measuring Farnesoid X Receptor Activity.- Cell-Based Assay for Identifying the Modulators of Antioxidant Response Element Signaling Pathway.- Study Liver Cytochrome P450 3A4 Inhibition and Hepatotoxicity Using DMSO-Differentiated HuH-7 Cells.- Determination of Histone H2AX Phosphorylation in DT40 Cells.- High-Throughput and High Content Micronucleus Assay in CHO-K1 Cells.- Better, Faster, Cheaper: Getting the Most Out of High-Throughput Screening with Zebrafish.- Fast Functional Germline and Epigenetic Assays in the Nematode .- A Quantitative High-Throughput Screening Data Analysis Pipeline for Activity Profiling.- Correction of Microplate Data from High-Throughput Screening.- CurveP Method for Rendering High-Throughput Screening Dose-Response Data into Digital Fingerprints.- Accounting Artifacts in High-Throughput Toxicity Assays.- Accessing the High-Throughput Screening Data Landscape.- Curating and Preparing High Throughput Screening Data for Quantitative Structure Activity Relationship Modeling.

Textul de pe ultima copertă

This book focuses on recently developed High Throughput Screening (HTS) assay protocols, many involved in the ToxCast and/or Tox21 initiatives, and the relevant HTS data analysis techniques. Divided into three sections, in vitro assays, in vivo assays, and computational techniques to analyze HTS data are all examined. Written for the highly successful Methods in Molecular Biology series, most chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, High Throughput Screening Assays in Toxicology serves as a valuable reference resource for translating new HTS techniques into standardized chemical toxicity assessment tools in order to advance modern toxicology research to a new era where HTS techniques can partially replace the prevailinganimal models.

Caracteristici

Includes cutting-edge methods and protocols for HTS assays to examine toxicity Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts Includes supplementary material: sn.pub/extras