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High Throughput Screening: Methods and Protocols: Methods in Molecular Biology, cartea 1439

Editat de William P. Janzen
en Limba Engleză Paperback – 31 mai 2018
This third edition volumeexpands on the previous editions both by presenting more detailed protocols forthe techniques described in the first and second editions of High ThroughputScreening: Methods and Protocolsand by covering important new procedures. The first chapter ofthis book provides an overview of important assay development techniques, whilethe rest of the chapters detail how to develop and execute screens at whateverthroughput the user needs. Some chapter examples are: structure-based virtualscreening,  high throughput screening using mass spectrometry, identification of state-dependentblockers for voltage gated calcium channels, bioluminescence resonanceenergy transfer platform to monitor protein-protein interactions in live cells,high throughput flow cytometry, and application of imaging-based assays in microplate formats for highcontent screening. Written in the highly successful Methods in MolecularBiology series format, chaptersinclude introductions to their respectivetopics, lists of the necessary materials and reagents, step-by-step, readilyreproducible laboratory protocols, and tips on troubleshooting and avoidingknown pitfalls.

Cutting edge and thorough, High Throughput Screening: Methods and Protocols,Third Edition, is a valuable resource for anyone who is interested in HTSresearch.
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Specificații

ISBN-13: 9781493981137
ISBN-10: 1493981137
Ilustrații: XI, 308 p. 101 illus., 71 illus. in color.
Dimensiuni: 178 x 254 mm
Greutate: 0.56 kg
Ediția:Softcover reprint of the original 3rd ed. 2016
Editura: Springer
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:New York, NY, United States

Cuprins

Design and Implementation of High Throughput Screening Assays.- Characterization of InhibitorBinding Through Multiple Inhibitor Analysis: A Novel Local Fitting Method.- High Throughput Screening UsingMass Spectrometry within Drug Discovery.- Structure-Based Virtual Screeningof Commercially Available Compound Libraries.- AlphaScreen-Based Assays:Ultra-High Throughput Screening for Small Molecule Inhibitors of ChallengingEnzymes and Protein-Protein Interactions.- Instrument Quality Control.- Application of FluorescencePolarization in HTS Assays.- Time Resolved Fluorescence Assays.- Protein Kinase SelectivityProfiling Using Microfluid Mobility Shift Assays.- Screening for Inhibitors ofKinase Autophosphorylation.- A Fluorescence-Based HighThroughput Screening Assay to Identify Growth Inhibitors of the PathogenicFungus Aspergillus fumigatus.- Mycobacterium tuberculosisHigh Throughput Screening.- Identification ofState-Dependent Blockers for Voltage Gated Calcium Channels Using a FLIPR BasedAssay.- A Luciferase Reporter GeneSystem for High Throughput Screening of γ-Globin GeneActivators.- A High Throughput Flow CytometryAssay for Identification of Inhibitors of 3’, 5’-Cyclic Adenosine MonophosphateEfflux.- High Throughput Cell ToxicityAssays.- BRETn: NanoLuc-BasedBioluminescence Resonance Energy Transfer Platform to Monitor Protein-ProteinInteractions in Live Cells.- Application of Imaging-BasedAssays in Microplate Formats for High Content Screening.


Textul de pe ultima copertă

This third edition volumeexpands on the previous editions both by presenting more detailed protocols forthe techniques described in the first and second editions of High ThroughputScreening: Methods and Protocolsand by covering important new procedures. The first chapter ofthis book provides an overview of important assay development techniques, whilethe rest of the chapters detail how to develop and execute screens at whateverthroughput the user needs. Some chapter examples are: structure-based virtualscreening,  high throughput screening using mass spectrometry, identification of state-dependentblockers for voltage gated calcium channels, bioluminescence resonanceenergy transfer platform to monitor protein-protein interactions in live cells,high throughput flow cytometry, and application of imaging-based assays in microplate formats for highcontent screening. Written in the highly successful Methods in MolecularBiology series format, chaptersinclude introductions to their respectivetopics, lists of the necessary materials and reagents, step-by-step, readilyreproducible laboratory protocols, and tips on troubleshooting and avoidingknown pitfalls.

Cutting edge and thorough, High Throughput Screening: Methods and Protocols,Third Edition, is a valuable resource for anyone who is interested in HTSresearch.

Caracteristici

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts