Cantitate/Preț
Produs
Total produse
Vezi coșul
Lentiviral Vectors and Exosomes as Gene and Protein Delivery Tools de Maurizio Federico

Lentiviral Vectors and Exosomes as Gene and Protein Delivery Tools: Methods in Molecular Biology, cartea 1448

Editat de Maurizio Federico
en Limba Engleză Hardback – 18 iun 2016
This volume provides current methods and protocols for geneand protein delivery based on both lentivirus-generated and spontaneouslyreleased nanovesicles.  Lentiviral Vectors and Exosomes as Gene andProtein Delivery Tools guides readers through methods on macromoleculedelivery and chapters describe the LV-based protocols of gene engineering. Written in thehighly successful Methods in Molecular Biology series format, chaptersinclude introductions to their respective topics, lists of the necessarymaterials and reagents, step-by-step, readily reproducible laboratoryprotocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Lentiviral Vectors andExosomes as Gene and Protein Delivery Tools aims to ensure successfulresults in the further study of this vital field.


Citește tot Restrânge

Toate formatele și edițiile

Toate formatele și edițiile Preț Express
Paperback (1) 71938 lei  6-8 săpt.
  Springer – 31 mai 2018 71938 lei  6-8 săpt.
Hardback (1) 72797 lei  6-8 săpt.
  Springer – 18 iun 2016 72797 lei  6-8 săpt.

Din seria Methods in Molecular Biology

  • 9% Nucleic Acid and Peptide Aptamers: Methods and Protocols
    Preț: 79159 lei
  • 23% Pheromone Signaling: Methods and Protocols
    Preț: 59856 lei
  • Engineering and Analyzing Multicellular Systems: Methods and Protocols
    Preț: 49679 lei
  • 20% Clinical Bioinformatics
    Preț: 88295 lei
  • Mitochondrial Regulation: Methods and Protocols
    Preț: 25204 lei
  • 5% Multiple Sclerosis: Methods and Protocols
    Preț: 72961 lei
  • 5% Bacterial Persistence: Methods and Protocols
    Preț: 73143 lei
  • 5% DNA-Protein Interactions: Principles and Protocols
    Preț: 74130 lei
  • 5% Methods in Mouse Atherosclerosis
    Preț: 74716 lei
  • 15% Cell Cycle Oscillators: Methods and Protocols
    Preț: 66345 lei
  • 18% TGF-β Signaling: Methods and Protocols
    Preț: 102534 lei
  • 5% Whole Genome Amplification: Methods and Protocols
    Preț: 73457 lei
  • 18% Patient-Specific Induced Pluripotent Stem Cell Models: Generation and Characterization
    Preț: 91420 lei
  • 15% Phospho-Proteomics: Methods and Protocols
    Preț: 66461 lei
  • 15% Candida Species: Methods and Protocols
    Preț: 65412 lei
  • 18% Induced Pluripotent Stem (iPS) Cells: Methods and Protocols
    Preț: 141474 lei
  • 5% Post-Transcriptional Gene Regulation
    Preț: 74260 lei
  • 20% In Vitro Embryogenesis in Higher Plants
    Preț: 82163 lei
  • 18% Yeast Functional Genomics: Methods and Protocols
    Preț: 97230 lei
  • 15% Statistical Analysis in Proteomics
    Preț: 66049 lei
  • 5% SiRNA Delivery Methods: Methods and Protocols
    Preț: 73841 lei
  • 18% Estrogen Receptors: Methods and Protocols
    Preț: 98492 lei
  • 5% Suppression and Regulation of Immune Responses: Methods and Protocols, Volume II
    Preț: 73329 lei
  • Plant Bioinformatics: Methods and Protocols
    Preț: 39258 lei
  • 5% Breast Cancer: Methods and Protocols
    Preț: 74626 lei
  • 18% Endocannabinoid Signaling: Methods and Protocols
    Preț: 96266 lei
  • 23% Zebrafish: Methods and Protocols
    Preț: 86021 lei
  • 15% Activity-Based Proteomics: Methods and Protocols
    Preț: 65264 lei
  • 5% Drug Target miRNA: Methods and Protocols
    Preț: 105550 lei
  • 23% Computational Protein Design
    Preț: 88385 lei
  • SH2 Domains: Methods and Protocols
    Preț: 79216 lei
  • Cholesterol Homeostasis: Methods and Protocols
    Preț: 42362 lei
  • 5% The Immune Synapse: Methods and Protocols
    Preț: 42591 lei
  • Organ Regeneration: 3D Stem Cell Culture & Manipulation
    Preț: 59220 lei
  • 5% Innate Antiviral Immunity: Methods and Protocols
    Preț: 34562 lei
  • 19% Protein Engineering: Methods and Protocols
    Preț: 49188 lei
  • 5% Innate Immune Activation: Methods and Protocols
    Preț: 103884 lei
  • 5% Investigations of Early Nutrition Effects on Long-Term Health: Methods and Applications
    Preț: 52415 lei
  • 18% Planarian Regeneration: Methods and Protocols
    Preț: 212234 lei
  • 5% Epitope Mapping Protocols
    Preț: 129923 lei
  • Tissue Proteomics: Methods and Protocols
    Preț: 78993 lei
  • 5% Genetic Epidemiology: Methods and Protocols
    Preț: 133910 lei
  • 18% Plant Chemical Genomics: Methods and Protocols
    Preț: 139026 lei
  • 5% Lung Innate Immunity and Inflammation: Methods and Protocols
    Preț: 75266 lei
  • 5% Rational Drug Design: Methods and Protocols
    Preț: 37489 lei
  • 18% Microbiome Analysis: Methods and Protocols
    Preț: 139563 lei
  • 18% Computational Methods in Protein Evolution
    Preț: 112965 lei
  • 18% Electrophoretic Separation of Proteins: Methods and Protocols
    Preț: 140826 lei
  • 18% Microbial Metabolomics: Methods and Protocols
    Preț: 112492 lei
  • 18% SNAREs: Methods and Protocols
    Preț: 96627 lei

Preț: 72797 lei

Preț vechi: 76628 lei
-5% Nou

Puncte Express: 1092
Preț estimativ în valută:
13936€ • 14541$ • 11682£

Carte tipărită la comandă

Livrare economică 13-27 martie

 Adaugă în coș

Wish list
Gift list
Am citit!
Adaugă în listă
Preluare comenzi: 021 569.72.76

Specificații

ISBN-13: 9781493937516
ISBN-10: 1493937510
Pagini: 40
Ilustrații: XI, 276 p. 47 illus., 33 illus. in color.
Dimensiuni: 178 x 254 x 18 mm
Greutate: 0.72 kg
Ediția:1st ed. 2016
Editura: Springer
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:New York, NY, United States

Cuprins

Construction of Modular Lentiviral Vectors for Effective Gene Expression and Knockdown.- Development of Inducible Molecular Switches Based on All-In-One Lentiviral Vectors Equipped With Drug Controlled FLP Recombinase.- Production Of Retrovirus-Based Vectors In Mildly Acidic Ph Conditions.- Optimized Lentiviral Transduction Protocols by use of a Poloxamer Enhancer, Spinoculation and Scfv-Antibody Fusions To VSV-G.- Transduction of Murine Hematopoietic Stem Cells with Tetracycline-Regulated Lentiviral Vectors.- Introduction of Shrnas, Mirnas or Antagomirs into Primary Human Liver Cells Through Lentiviral Vectors.- Production and Concentration of Lentivirus for Transduction of Primary Human T Cells.- Generating Transgenic Mice by Lentiviral Transduction of Spermatozoa Followed by In Vitro Fertilization and Embryo Transfer.- The LAM-PCR Method to Sequence LV Integration Sites.- Conditional Rnai using the Lentiviral GLTR System.- Lentiviral Vectors for the Engineering of Implantable Cells Secreting Recombinant Antibodies.- Transient Expression of Green Fluorescent Protein in Integrase-Defective Lentiviral Vector Transduced 293T Cell Line.- Intrastriatal Delivery of Integration-Deficient Lentiviral Vectors in a Rat Model of Parkinson's Disease.- Development of Lentiviral Vectors for Targeted Integration and Protein Delivery.- Biogenesis and Functions of Exosomes and Extracellular Vesicles.- Generation, Quantification, and Tracing of Metabolically Labeled Fluorescent Exosomes.- Cardiac Myocyte Exosome Isolation.- Incorporation of Heterologous Proteins in Engineered Exosomes.- Exosome-Mediated Targeted Delivery of Mirnas.







Textul de pe ultima copertă

This volume provides current methods and protocols for gene and protein delivery based on both lentivirus-generated and spontaneously released nanovesicles.  Lentiviral Vectors and Exosomes as Gene and Protein Delivery Tools guides readers through methods on macromolecule delivery and chapters describe the LV-based protocols of gene engineering. Written in the highly successful Methods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Lentiviral Vectors and Exosomes as Gene and Protein Delivery Tools aims to ensure successful results in the further study of this vital field.

Caracteristici

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts