Lentiviral Vectors and Exosomes as Gene and Protein Delivery Tools de Maurizio Federico

Lentiviral Vectors and Exosomes as Gene and Protein Delivery Tools: Methods in Molecular Biology, cartea 1448

Maurizio Federico

This volume provides current methods and protocols for geneand protein delivery based on both lentivirus-generated and spontaneouslyreleased nanovesicles. Lentiviral Vectors and Exosomes as Gene andProtein Delivery Tools guides readers through methods on macromoleculedelivery and chapters describe the LV-based protocols of gene engineering. Written in thehighly successful Methods in Molecular Biology series format, chaptersinclude introductions to their respective topics, lists of the necessarymaterials and reagents, step-by-step, readily reproducible laboratoryprotocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Lentiviral Vectors andExosomes as Gene and Protein Delivery Tools aims to ensure successfulresults in the further study of this vital field.

Citește tot Restrânge

Toate formatele și edițiile

	Preț	Express
Paperback (1)	704^.83 lei 43-57 zile
Springer – 31 mai 2018	704^.83 lei 43-57 zile
Hardback (1)	713^.25 lei 43-57 zile
Springer – 18 iun 2016	713^.25 lei 43-57 zile

Din seria Methods in Molecular Biology

60%

Preț: 347^.95 lei
23%

Preț: 598^.56 lei
Preț: 496^.79 lei
20%

Preț: 882^.95 lei
Preț: 252^.04 lei
5%

Preț: 714^.88 lei
5%

Preț: 716^.65 lei
5%

Preț: 726^.33 lei
5%

Preț: 732^.06 lei
15%

Preț: 650^.04 lei
18%

Preț: 1004^.55 lei
5%

Preț: 719^.71 lei
18%

Preț: 895^.67 lei
15%

Preț: 651^.17 lei
15%

Preț: 640^.89 lei
18%

Preț: 1386^.03 lei
5%

Preț: 727^.59 lei
20%

Preț: 821^.63 lei
18%

Preț: 952^.59 lei
15%

Preț: 647^.15 lei
5%

Preț: 723^.48 lei
18%

Preț: 964^.97 lei
5%

Preț: 718^.46 lei
Preț: 392^.58 lei
5%

Preț: 731^.17 lei
18%

Preț: 943^.15 lei
23%

Preț: 860^.21 lei
15%

Preț: 639^.44 lei
5%

Preț: 1034^.11 lei
23%

Preț: 883^.85 lei
Preț: 792^.16 lei
Preț: 423^.62 lei
5%

Preț: 425^.91 lei
Preț: 592^.20 lei
5%

Preț: 345^.62 lei
19%

Preț: 491^.88 lei
5%

Preț: 1038^.84 lei
5%

Preț: 524^.15 lei
18%

Preț: 2079^.22 lei
5%

Preț: 1272^.89 lei
Preț: 789^.93 lei
5%

Preț: 1339^.10 lei
18%

Preț: 1362^.07 lei
5%

Preț: 752^.66 lei
5%

Preț: 374^.89 lei
18%

Preț: 1367^.31 lei
18%

Preț: 1106^.74 lei
18%

Preț: 1379^.69 lei
18%

Preț: 1102^.11 lei
18%

Preț: 946^.69 lei

Cuprins

Construction of Modular Lentiviral Vectors for Effective Gene Expression and Knockdown.- Development of Inducible Molecular Switches Based on All-In-One Lentiviral Vectors Equipped With Drug Controlled FLP Recombinase.- Production Of Retrovirus-Based Vectors In Mildly Acidic Ph Conditions.- Optimized Lentiviral Transduction Protocols by use of a Poloxamer Enhancer, Spinoculation and Scfv-Antibody Fusions To VSV-G.- Transduction of Murine Hematopoietic Stem Cells with Tetracycline-Regulated Lentiviral Vectors.- Introduction of Shrnas, Mirnas or Antagomirs into Primary Human Liver Cells Through Lentiviral Vectors.- Production and Concentration of Lentivirus for Transduction of Primary Human T Cells.- Generating Transgenic Mice by Lentiviral Transduction of Spermatozoa Followed by In Vitro Fertilization and Embryo Transfer.- The LAM-PCR Method to Sequence LV Integration Sites.- Conditional Rnai using the Lentiviral GLTR System.- Lentiviral Vectors for the Engineering of Implantable Cells Secreting Recombinant Antibodies.- Transient Expression of Green Fluorescent Protein in Integrase-Defective Lentiviral Vector Transduced 293T Cell Line.- Intrastriatal Delivery of Integration-Deficient Lentiviral Vectors in a Rat Model of Parkinson's Disease.- Development of Lentiviral Vectors for Targeted Integration and Protein Delivery.- Biogenesis and Functions of Exosomes and Extracellular Vesicles.- Generation, Quantification, and Tracing of Metabolically Labeled Fluorescent Exosomes.- Cardiac Myocyte Exosome Isolation.- Incorporation of Heterologous Proteins in Engineered Exosomes.- Exosome-Mediated Targeted Delivery of Mirnas.

Textul de pe ultima copertă

This volume provides current methods and protocols for gene and protein delivery based on both lentivirus-generated and spontaneously released nanovesicles. Lentiviral Vectors and Exosomes as Gene and Protein Delivery Tools guides readers through methods on macromolecule delivery and chapters describe the LV-based protocols of gene engineering. Written in the highly successful Methods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Lentiviral Vectors and Exosomes as Gene and Protein Delivery Tools aims to ensure successful results in the further study of this vital field.

Lentiviral Vectors and Exosomes as Gene and Protein Delivery Tools: Methods in Molecular Biology, cartea 1448

Toate formatele și edițiile

Din seria Methods in Molecular Biology

Preț: 713^.25 lei

Carte tipărită la comandă

Specificații

Cuprins

Textul de pe ultima copertă

Caracteristici

Papetărie, jocuri, reviste

Lentiviral Vectors and Exosomes as Gene and Protein Delivery Tools: Methods in Molecular Biology, cartea 1448

Toate formatele și edițiile

Preț: 713.25 lei

Specificații

V-ar putea interesa

Cuprins

Textul de pe ultima copertă

Caracteristici

Preț: 713^.25 lei