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Lentiviral Vectors and Exosomes as Gene and Protein Delivery Tools de Maurizio Federico

Lentiviral Vectors and Exosomes as Gene and Protein Delivery Tools: Methods in Molecular Biology, cartea 1448

Editat de Maurizio Federico
en Limba Engleză Hardback – 18 iun 2016
This volume provides current methods and protocols for geneand protein delivery based on both lentivirus-generated and spontaneouslyreleased nanovesicles.  Lentiviral Vectors and Exosomes as Gene andProtein Delivery Tools guides readers through methods on macromoleculedelivery and chapters describe the LV-based protocols of gene engineering. Written in thehighly successful Methods in Molecular Biology series format, chaptersinclude introductions to their respective topics, lists of the necessarymaterials and reagents, step-by-step, readily reproducible laboratoryprotocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Lentiviral Vectors andExosomes as Gene and Protein Delivery Tools aims to ensure successfulresults in the further study of this vital field.


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Specificații

ISBN-13: 9781493937516
ISBN-10: 1493937510
Pagini: 40
Ilustrații: XI, 276 p. 47 illus., 33 illus. in color.
Dimensiuni: 178 x 254 x 18 mm
Greutate: 0.72 kg
Ediția:1st ed. 2016
Editura: Springer
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:New York, NY, United States

Cuprins

Construction of Modular Lentiviral Vectors for Effective Gene Expression and Knockdown.- Development of Inducible Molecular Switches Based on All-In-One Lentiviral Vectors Equipped With Drug Controlled FLP Recombinase.- Production Of Retrovirus-Based Vectors In Mildly Acidic Ph Conditions.- Optimized Lentiviral Transduction Protocols by use of a Poloxamer Enhancer, Spinoculation and Scfv-Antibody Fusions To VSV-G.- Transduction of Murine Hematopoietic Stem Cells with Tetracycline-Regulated Lentiviral Vectors.- Introduction of Shrnas, Mirnas or Antagomirs into Primary Human Liver Cells Through Lentiviral Vectors.- Production and Concentration of Lentivirus for Transduction of Primary Human T Cells.- Generating Transgenic Mice by Lentiviral Transduction of Spermatozoa Followed by In Vitro Fertilization and Embryo Transfer.- The LAM-PCR Method to Sequence LV Integration Sites.- Conditional Rnai using the Lentiviral GLTR System.- Lentiviral Vectors for the Engineering of Implantable Cells Secreting Recombinant Antibodies.- Transient Expression of Green Fluorescent Protein in Integrase-Defective Lentiviral Vector Transduced 293T Cell Line.- Intrastriatal Delivery of Integration-Deficient Lentiviral Vectors in a Rat Model of Parkinson's Disease.- Development of Lentiviral Vectors for Targeted Integration and Protein Delivery.- Biogenesis and Functions of Exosomes and Extracellular Vesicles.- Generation, Quantification, and Tracing of Metabolically Labeled Fluorescent Exosomes.- Cardiac Myocyte Exosome Isolation.- Incorporation of Heterologous Proteins in Engineered Exosomes.- Exosome-Mediated Targeted Delivery of Mirnas.







Textul de pe ultima copertă

This volume provides current methods and protocols for gene and protein delivery based on both lentivirus-generated and spontaneously released nanovesicles.  Lentiviral Vectors and Exosomes as Gene and Protein Delivery Tools guides readers through methods on macromolecule delivery and chapters describe the LV-based protocols of gene engineering. Written in the highly successful Methods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Lentiviral Vectors and Exosomes as Gene and Protein Delivery Tools aims to ensure successful results in the further study of this vital field.

Caracteristici

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts