Cantitate/Preț
Produs

Mammalian Chromosome Engineering: Methods and Protocols: Methods in Molecular Biology, cartea 738

Editat de Gyula Hadlaczky
en Limba Engleză Paperback – 23 aug 2016
The rapid progression of genetics and molecular biology has turned chromosomal engineering from science fiction to reality, with the successful production of transgenic animals with engineered chromosomes and chromosomes developed for pharmaceutical protein production which are now ready for the medical industry.  Mammalian Chromosome Engineering: Methods and Protocols provides the reader with up-to date information on this rapidly evolving field and strives to take the reader into the exciting realm of chromosomal engineering from the basic principles to the practical applications of these new technologies. The five overview and ten protocol chapters cover the engineering of chromosomes with extrachromosomal vectors and transposon systems, the manipulation of naturally occurred minichromosomes, the generation and engineering of synthetic artificial chromosomes, and the induced de novo platform artificial chromosome system.  Written in the highly successful Methods in Molecular Biology™ series format, protocols chapters contain brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
 
Authoritative and cutting-edge, Mammalian Chromosome Engineering: Methods and Protocols serves as a bench-side resource for current protocols and aims to help scientists to explore the many prospects for future research and vital applications.
Citește tot Restrânge

Toate formatele și edițiile

Toate formatele și edițiile Preț Express
Paperback (1) 88271 lei  6-8 săpt.
  Humana Press Inc. – 23 aug 2016 88271 lei  6-8 săpt.
Hardback (1) 71077 lei  6-8 săpt.
  Humana Press Inc. – 6 apr 2011 71077 lei  6-8 săpt.

Din seria Methods in Molecular Biology

Preț: 88271 lei

Preț vechi: 92918 lei
-5% Nou

Puncte Express: 1324

Preț estimativ în valută:
16899 17565$ 14011£

Carte tipărită la comandă

Livrare economică 05-19 februarie 25

Preluare comenzi: 021 569.72.76

Specificații

ISBN-13: 9781493957750
ISBN-10: 1493957759
Pagini: 257
Ilustrații: IX, 257 p.
Dimensiuni: 178 x 254 mm
Greutate: 0.47 kg
Ediția:Softcover reprint of the original 1st ed. 2011
Editura: Humana Press Inc.
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:Totowa, NJ, United States

Cuprins

Developing Extrachromosomal Gene Expression Vector Technologies: An Overview.- High Capacity Extrachromosomal Gene Expression Vectors.- Naturally Occurring Minichromosome Platforms in Chromosome Engineering: An Overview.- Chromosome Transfer Via Cell Fusion.- Insertional Engineering of Chromosomes with Sleeping Beauty Transposition: An Overview.- Rodent Transgenesis Mediated by a Novel Hyperactive Sleeping Beauty Transposon System.- Construction and Use of a Bottom-Up HAC Vector for Transgene Expression.- De novo Generation of Satellite DNA-Based Artificial Chromosomes by Induced Large-Scale Amplification.- Downstream Bioengineering of ACE Chromosomes for Incorporation of Site-Specific Recombination Cassettes.- Chromosome Engineering with Lambda-Integrase Mediated Recombination System: The ACE System.- Dendrimer Mediated Transfer of Engineered Chromosomes.- Engineered Chromosomes in Transgenics.- Transfer of Stem Cells Carrying Engineered Chromosomes with XY Clone Laser System.- Mammalian Artificial Chromosomes and Clinical Applications for Genetic Modification of Stem Cells: An Overview.- Engineered Mammalian Chromosomes in Cellular Protein Production: Future Prospects.

Textul de pe ultima copertă

The rapid progression of genetics and molecular biology has turned chromosomal engineering from science fiction to reality, with the successful production of transgenic animals with engineered chromosomes and chromosomes developed for pharmaceutical protein production which are now ready for the medical industry.  Mammalian Chromosome Engineering: Methods and Protocols provides the reader with up-to date information on this rapidly evolving field and strives to take the reader into the exciting realm of chromosomal engineering from the basic principles to the practical applications of these new technologies. The five overview and ten protocol chapters cover the engineering of chromosomes with extrachromosomal vectors and transposon systems, the manipulation of naturally occurred minichromosomes, the generation and engineering of synthetic artificial chromosomes, and the induced de novo platform artificial chromosome system.  Written in the highly successful Methods in Molecular Biology™ series format, protocols chapters contain brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
 
Authoritative and cutting-edge, Mammalian Chromosome Engineering: Methods and Protocols serves as a bench-side resource for current protocols and aims to help scientists to explore the many prospects for future research and vital applications.

Caracteristici

Provides a precious resource for scientists interested in new ideas in gene technology Features practical protocols from the cutting-edge of this fast-paced field Includes expert tips and key implementation advice crucial for getting optimal results Includes supplementary material: sn.pub/extras