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Metabolomics: Methods and Protocols de Wolfram Weckwerth

Metabolomics: Methods and Protocols: Methods in Molecular Biology, cartea 358

Editat de Wolfram Weckwerth
en Limba Engleză Paperback – 19 noi 2010
Metabolomics: Methods and Protocols examines the state-of-the-art in metabolomic analysis. Leading researchers in the field present protocols for the application of complementary analytical methods, such as gas chromatography-mass spectrometry (GC-MS). Metabolomics: Methods and Protocols contains forward-looking protocols, which provide the essential groundwork for future efforts in elucidating the structure of the unknowns detected in metabolomic studies.
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Specificații

ISBN-13: 9781617376436
ISBN-10: 1617376434
Pagini: 312
Ilustrații: 312 p. 79 illus., 8 illus. in color.
Dimensiuni: 152 x 229 x 18 mm
Greutate: 0.41 kg
Ediția:Softcover reprint of hardcover 1st ed. 2007
Editura: Humana Press Inc.
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:Totowa, NJ, United States

Public țintă

Research

Cuprins

Metabolome Analysis Using Gas Chromatography Coupled to Mass Spectrometry.- Metabolite Profiling in Blood Plasma.- Nonsupervised Construction and Application of Mass Spectral and Retention Time Index Libraries From Time-of-Flight Gas Chromatography-Mass Spectrometry Metabolite Profiles.- Metabolomic Profiling of Natural Volatiles.- Metabolomics: Data Integration and Data Mining.- Integrative Profiling of Metabolites and Proteins.- Integrating Profiling Data.- Visualization and Analysis of Molecular Data.- A Gentle Guide to the Analysis of Metabolomic Data.- Capillary Electrophoresis Coupled to Mass Spectrometry.- Capillary Electrophoresis-Mass Spectrometry for Metabolomics.- Liquid Chromatography Coupled to Mass Spectrometry for Metabolomics and Structural Elucidation.- Application of Liquid Chromatography-Mass Spectrometry Analysis in Metabolomics.- Electrochemical Detection of Metabolites.- High-Performance Liquid Chromatography Separations Coupled With Coulometric Electrode Array Detectors.- Metabolic Fluxes.- Determination of Metabolic Flux Ratios From 13C-Experiments and Gas Chromatography-Mass Spectrometry Data.- Understanding the Roadmap of Metabolism by Pathway Analysis.- NMR Metabolome Analysis.- Revealing the Metabolome of Animal Tissues Using 1H Nuclear Magnetic Resonance Spectroscopy.- Nuclear Magnetic Resonance Metabonomics.- Practical Aspects of Uniform Stable Isotope Labeling of Higher Plants for Heteronuclear NMR-Based Metabolomics.

Recenzii

From the reviews:
"This book forms part of an extensive and successful series from Methods in Molecular Biology … . this book provides a good introduction to metabolomics, with useful protocols and relevant data analysis techniques that can be adapted easily to suit any researcher’s needs." (Elizabeth Want, ChemMedChem, Issue 2, 2007)

Textul de pe ultima copertă

Metabolomics: Methods and Protocols examines the state-of-the-art in metabolomic analysis. Leading researchers in the field present protocols for the application of complementary analytical methods, such as gas chromatography-mass spectrometry (GC-MS). Metabolomics: Methods and Protocols contains forward-looking protocols, which provide the essential groundwork for future efforts in elucidating the structure of the unknowns detected in metabolomic studies.

Part I of this volume focuses on GC-MS techniques; specifically, attention is given to metabolite profiling in blood plasma, construction and application of mass spectral and retention time index libraries, and metabolomic profiling of natural volatiles. In Part II, emphasis shifts to data integration and data mining techniques, and specific protocols discuss improving pattern recognition and biomarker selection for systems level approaches, and visualization and analysis of molecular and metabolomic data. Parts III, IV, and V examine the coupling of capillary electrophoresis and mass spectrometry, classical reversed phase liquid chromatography-mass spectrometry, and electrochemical detection of metabolites using HPLC separations coupled with coulometric electrode array detectors. In Part VI, protocols for the determination of metabolic flux rates are presented and a theoretical framework for metabolic pathway network analysis is provided. The volume concludes with an examination of cutting-edge NMR protocols.

Caracteristici

Includes supplementary material: sn.pub/extras