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Methicillin-Resistant Staphylococcus Aureus (MRSA) Protocols: Cutting-Edge Technologies and Advancements: Methods in Molecular Biology, cartea 2069

Editat de Yinduo Ji
en Limba Engleză Paperback – 16 sep 2020
This third edition volume expands on the previous editions with an update on the latest techniques used for the detection, genotyping, and investigating pathogenesis of Staphylococcus aureus in vitro and in vivo. The methods covered in this book mostly focus on routine clinical diagnosis, surveillance, research, and practice for treatment of patients infected by multi-drug resistant S. aureus. The book also covers the epidemiology of MRSA, molecular typing approaches, clinical treatment of MRSA infections, and animal models of drug discovery. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Informative and cutting-edge, Methicillin-Resistant Staphylococcus Aureus (MRSA) Protocols: Cutting-Edge Technologies and Advancements, Third Edition is a valuable resource for researchers looking to set up new methods to study S. aureus, and will also be very useful for technicians and scientists working on other bacterial pathogens. 

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Specificații

ISBN-13: 9781493998517
ISBN-10: 149399851X
Pagini: 268
Ilustrații: XI, 268 p. 35 illus., 27 illus. in color.
Dimensiuni: 178 x 254 mm
Ediția:3rd ed. 2020
Editura: Springer Us
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:New York, NY, United States

Cuprins

Clinical, Epidemiologic, and Laboratory Aspects of Methicillin-Resistant Staphylococcus aureus Infections.- Rapid Methods for Detection of MRSA in Clinical Specimens.- Immunofluorescence Microscopy for the Detection of Surface Antigens in Methicillin Resistant Staphylococcus aureus.- Staphylococcal Cassette Chromosome mec (SCCmec) Analysis of MRSA.- Pulsed-Field Gel Electrophoresis Typing of Staphylococcus aureus Strains.- Spa Genotyping of Staphylococcus aureus Isolates.- Multilocus Sequence Typing of Staphylococcus aureus.- Genetic Manipulations of Staphylococcal Chromosomal DNA.- Genetic Manipulation of MRSA using CRISPR/Cas9 Technology.-  RNA-seq Identification of Target Genes Mediated by Regulators of Staphylococcus aureus.- Application of Two-Dimensional Difference Gel Electrophoresis in Identification of Factors Responsible for Virulence of Staphylococcus aureus.- Identification of Virulence Determinants during Host-Pathogen Interaction using Tn-Seq Technology.- Metabolomic Profiling of Staphylococcus aureus.- Determining Impact of Growth Phases on Capacity of Staphylococcus aureus to Adhere to and Invade Host Cells.- Preclinical Models and Methodologies for Monitoring Staphylococcus aureus Infections using Noninvasive Optical Imaging.- Methicillin-Resistant Staphylococcus aureus Infection and Treatment Options.- Animal Models for Drug Development for MRSA.



Textul de pe ultima copertă

This third edition volume expands on the previous editions with an update on the latest techniques used for the detection, genotyping, and investigating pathogenesis of Staphylococcus aureus in vitro and in vivo. The methods covered in this book mostly focus on routine clinical diagnosis, surveillance, research, and practice for treatment of patients infected by multi-drug resistant S. aureus. The book also covers the epidemiology of MRSA, molecular typing approaches, clinical treatment of MRSA infections, and animal models of drug discovery. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Informative and cutting-edge,  Methicillin-Resistant Staphylococcus Aureus (MRSA) Protocols: Cutting-Edge Technologies and Advancements, Third Edition is a valuable resource for researchers looking to set up new methods to study S. aureus, and will also be very useful for technicians and scientists working on other bacterial pathogens. 


Caracteristici

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Recenzii

From the reviews:
"This book … has a unique format focussing on detailed experimental protocols. This is particularly useful if you are trying a technique for the first time, troubleshooting a method, or wishing to understand how a technique works in order to critique its use in the literature. … there is much to recommend in this book, particularly those who are new to the field and want an introduction to techniques in both basic biology and clinical aspects of MRSA." (Jodi Lindsay, Microbiology Today, May, 2008)
"The book is devoted to the multiple laboratory techniques available for technicians and basic scientists. … a good field manual, and clinicians will have an easy to read book on these techniques. … describes well the laboratory techniques available for the study of MRSA. … Most chapters have illustrations in black and white, which are helpful in the overall discussion, and all chapters have a bibliographic list. … it is a good starting point for clinicians and novice scientists in the world of MRSA infections." (Carlos Ernesto Figueroa Castro, Doody’s Review Service, May, 2008)
"A unique compilation of 17 chapters aimed chiefly at the scientific investigation of MRSA … . This book may also serve as a classic methods reference for those geared towards studying MRSA … . In summary, this book represents an excellent reference manual of protocols used to study MRSA, with concise, clear, and well-written introductory sections that can be useful for laboratory investigators and clinicians wishing to get a better understanding of the theory behind scientific methods. … is timely in its arrival." (George Sakoulas, Clinical Infectious Diseases, Vol. 46, May, 2008)