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Mitochondrial Medicine: Volume 3: Manipulating Mitochondria and Disease- Specific Approaches de Volkmar Weissig

Mitochondrial Medicine: Volume 3: Manipulating Mitochondria and Disease- Specific Approaches: Methods in Molecular Biology, cartea 2277

Editat de Volkmar Weissig, Marvin Edeas
en Limba Engleză Paperback – 3 iun 2021
This second edition offers 88 chapters divided among three volumes providing the most comprehensive source of know-how in the wide-ranging field of Mitochondrial Medicine. Volume III guides readers through chapters on intercellular mitochondrial transfer, Cytoplasmic transfer methods, Ndufs4 knockout mouse, in vivo assessment of mitochondrial oxygen consumption, mitochondrial network analysis by imaging, mitochondrial respiration in platelets, Heterologous Inferential Analysis (HIA), bioenergetic profiling, integrative methods for studying cardiac energetics, and isolation and deep sequencing of mitochondrial DNA.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls.
 Authoritative and accessible, Mitochondrial Medicine, Second Edition, Volume 3: Manipulating Mitochondria and Disease- Specific Approaches aims to be a comprehensive source of know-how in the wide-ranging field of Mitochondrial Medicine.
 
 
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Specificații

ISBN-13: 9781071612729
ISBN-10: 1071612727
Pagini: 468
Ilustrații: XV, 468 p. 92 illus., 60 illus. in color.
Dimensiuni: 178 x 254 x 29 mm
Greutate: 0.84 kg
Ediția:2nd ed. 2021
Editura: Springer Us
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:New York, NY, United States

Cuprins

Allotopic Expression of ATP6 in the Mouse as a Transgenic Model of Mitochondrial Disease.- Mitochondrial Transplantation for Ischemia Reperfusion Injury.- Quantitatively Controlled Intercellular Mitochondrial Transfer by Cell Fusion-Based Method Using a Microfluidic Device.- Lipophilic Conjugates for Carrier-Free Delivery of RNA Importable Into Human Mitochondria.- Drug Discovery Assay to Identify Modulators of the Mitochondrial Ca2+ Uniporter.- Cytoplasmic Transfer Methods for Studying the Segregation of Mitochondrial DNA in Mice.- Electron Attachment to Isolated Molecules as a Probe To Understand Mitochondrial Reductive Processes.- Assessment of Short- and Medium-Chain Fatty Acids on Mitochondrial Function in Severe Inflammation.- Assessment of the Effects of Drugs on Mitochondrial Respiration.- The Ndufs4 Knockout Mouse: A Dual Threat Model of Childhood Mitochondrial Disease and Normative Aging.- Suborganellar Localisation of Mitochondrial Proteins and Transcripts in Human Cells.- In vivo Assessment of Mitochondrial Oxygen Consumption.- Update of Mitochondrial Network Analysis by Imaging: Proof of Technique in Schizophrenia.- Heterologous Inferential Analysis (HIA) and other Emerging Concepts In Understanding Mitochondrial Variation in Pathogenesis: There is no More Low Hanging Fruit.- Accurate Measurement of Cellular and Cell-Free Circulating Mitochondrial DNA Content from Human Blood Samples Using Real Time Quantitative PCR.- Measurement of Mitochondrial Respiration in Platelets.- Isolation and Electron Microscopic Analysis of Liver Cancer Cell Mitochondria.- Assessment of Mitochondrial Reactive Oxygen Species and Redox Regulation in Stem Cells.- Single Cell Approaches for Studying the Role of Mitochondrial DNA in Neurodegenerative Disease.- A New Method for Sequencing the Mitochondrial Genome by Using Long Read Technology.- Mitochondrial DNA as a Sensitive Biomarker of UV-induced Cellular Damage in Human Skin.- Isolation of Mitochondria-associated ER Membranes (MAMs), Synaptic MAMs and Glycosphingolipid Enriched Microdomains (GEMs) from Brain Tissues and Neuronal Cells.- Mitochondrial Diagnostics: A Discovery-Based Biochemical Platform for Phenotyping Human Peripheral Blood Cell Mitochondria.- Bioenergetic Profiling Of Human Pluripotent Stem Cells.- Integrative Methods for Studying Cardiac Energetics.- Isolation of Mitochondria from Retinal Pigment Epithelial Cell Cultures and an Application Of High Resolution Respirometric Assay (XFe96 Seahorse assay).- The Isolation and Deep Sequencing of Mitochondrial DNA.- Ultrastructure of the Mitochondria-Associated Membranes in Human Tumor Specimens.
 

Textul de pe ultima copertă

This second edition offers 88 chapters divided among three volumes providing the most comprehensive source of know-how in the wide-ranging field of Mitochondrial Medicine. Volume III guides readers through chapters on intercellular mitochondrial transfer, Cytoplasmic transfer methods, Ndufs4 knockout mouse, in vivo assessment of mitochondrial oxygen consumption, mitochondrial network analysis by imaging, mitochondrial respiration in platelets, Heterologous Inferential Analysis (HIA), bioenergetic profiling, integrative methods for studying cardiac energetics, and isolation and deep sequencing of mitochondrial DNA.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and accessible, Mitochondrial Medicine, Second Edition, Volume 3: Manipulating Mitochondria and Disease- Specific Approaches aims to be a comprehensive source of know-how in the wide-ranging field of Mitochondrial Medicine.


Caracteristici

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts