Cantitate/Preț
Produs

Transposons and Retrotransposons: Methods and Protocols: Methods in Molecular Biology, cartea 1400

Editat de Jose Luis Garcia Perez
en Limba Engleză Paperback – 31 mar 2018
This volume covers the latest protocols designed to identify and characterize TEs in genomes, ancient or recently inserted. Additionally, this book includes a series of protocols designed to understand how host genomes act to regulate the activity of TEs, from elegant genetic mobilization assays to key biochemical methods. Finally, this book also includes chapters that describe how TEs can be used for biotechnological applications. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Transposons and Retrotransposons: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Citește tot Restrânge

Toate formatele și edițiile

Toate formatele și edițiile Preț Express
Paperback (1) 77702 lei  43-57 zile
  Springer – 31 mar 2018 77702 lei  43-57 zile
Hardback (1) 72383 lei  43-57 zile
  Springer – 20 feb 2016 72383 lei  43-57 zile

Din seria Methods in Molecular Biology

Preț: 77702 lei

Preț vechi: 81792 lei
-5% Nou

Puncte Express: 1166

Preț estimativ în valută:
14871 15447$ 12352£

Carte tipărită la comandă

Livrare economică 03-17 februarie 25

Preluare comenzi: 021 569.72.76

Specificații

ISBN-13: 9781493980338
ISBN-10: 1493980335
Ilustrații: XII, 434 p. 73 illus., 56 illus. in color.
Dimensiuni: 178 x 254 mm
Greutate: 0.77 kg
Ediția:Softcover reprint of the original 1st ed. 2016
Editura: Springer
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:New York, NY, United States

Cuprins

Study of Transposable Elements and their Genomic Impact.- Bacterial Group II Introns: Identification and Mobility Assay.- In silico Methods to Identify Exapted Transposable Element Families.- Retrotransposon Capture Sequencing (RC-seq): A Targeted, High-Throughput Approach to Resolve Somatic L1 Retrotransposition in Humans.- Long Interspersed Element Sequencing (L1-Seq): A Method to Identify Somatic LINE-1 Insertions in the Human Genome.- Combining Amplification Typing of L1 Active Subfamilies (ATLAS) with High Throughput Sequencing.- RNA-seq Analysis to Measure the Expression of SINE Retroelements.- Qualitative and Quantitative Assays of Transposition and Homologous Recombination of the Retrotransposon Tf1 in Schizosaccharomyces pombe.- LINE Retrotransposition Assays in Saccharomyces cerevisiae.- LINE-1 Cultured CellRetrotransposition Assay.- L1 Retrotransposition in Neural Progenitor Cells.- Characterization of Engineered L1 Retrotransposition Events: The Recovery Method.- SINE Retrotransposition: Evaluation of Alu Activity and Recovery of de novo Inserts.- The Engineered SVA Trans-Mobilization Assay.- Detection of LINE-1 RNAs by Northern Blot.- Monitoring Long Interspersed Nuclear Element 1 Expression during Mouse Embryonic Stem Cells Differentiation.- Immuno-Detection of Human LINE-1 Expression in Cultured Cells and Human Tissues.- Cellular Localization of Engineered Human LINE-1 RNA and Proteins.- Purification of L1-ribonucleoprotein Particles (L1-RNPs) from Cultured Human Cells.- Characterization of L1-RiboNucleoprotein Particles.- LEAP: L1 Element Amplification Protocol.- Biochemical Approaches to Study LINE-1 Reverse Transcriptase Activity In Vitro.- Methylated DNA Immunoprecipitation Analysis of Mammalian Endogenous Retroviruses.- Profiling DNA Methylation and Hydroxymethylation at Retrotransposable Elements.- A Large-Scale Functional Screen to Identify Epigenetic Repressors of Retrotransposon Expression.- Reprogramming of Human Fibroblasts to Induced Pluripotent Stem Cells with Sleeping Beauty Transposon-based Stable Gene Delivery.
 

Caracteristici

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts