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Plant Kinases: Methods and Protocols: Methods in Molecular Biology, cartea 779

Editat de Nico Dissmeyer, Arp Schnittger
en Limba Engleză Hardback – 26 aug 2011
Modification of target protein properties by reversible phosphorylation events has been found to be one of the most prominent cellular control processes in all organisms. Recent advances in the areas of molecular biology and biochemistry are presenting new possibilities for reaching an unprecedented depth and a proteome-wide understanding of phosphorylation processes in plants as well as in other species. The major goal of Plant Kinases: Methods and Protocols is to provide the experimentalist with a detailed account of the practical steps necessary for successfully carrying out each protocol in his or her own laboratory. Plant protein kinases specifically addressed in this volume are members of the plant MAP kinase cascade, cyclin- and Calcium-dependent protein kinases, and plant sensor and receptor kinases. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
 
Authoritative and accessible, Plant Kinases: Methods and Protocols will prove a useful laboratory companion to  both novice and seasoned researchers by facilitating the practical work that will lead them to new and exciting insights in this dynamic field.
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Specificații

ISBN-13: 9781617792632
ISBN-10: 1617792632
Pagini: 285
Ilustrații: X, 312 p.
Dimensiuni: 178 x 254 x 23 mm
Greutate: 0.78 kg
Ediția:2011
Editura: Humana Press Inc.
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:Totowa, NJ, United States

Public țintă

Professional/practitioner

Cuprins

Guide to the Book Plant Kinases.- The Age of Protein Kinases.- Expression and Purification of Active Protein Kinases from Wheat Germ Extracts.- Measurement of Plant Cyclin-Dependent Kinase Activity Using Immunoprecipitation-Coupled and Affinity Purification-Based Kinase Assays and the Baculovirus Expression System.- Mitogen-Activated Protein Kinase Activity and Reporter Gene Assays in Plants.- Use of Phospho-site Substitutions to Analyze the Biological Relevance of Phosphorylation Events in Regulatory Networks.- Bacterial Assay to Study Plant Sensor Histidine Kinases.-  Substrate Analysis of Arabidopsis PP2C-type Protein Phosphatase.- Modulating and Monitoring MAPK Activity During Programmed Cell Death in Pollen.- Sensitizing Plant Protein Kinases to Specific Inhibition by ATP-Competitive Molecules.- Fluorescence Fluctuation Analysis of Receptor Kinase Dimerization.- Quantifying Degradation Rates of Trans-Membrane Receptor Kinases.- Fluorescence Correlation Spectroscopy and Fluorescence Recovery after Photobleaching to Study Receptor Kinase Mobility in planta.- Bimolecular-Fluorescence Complementation Assay to Monitor Kinase-Substrate Interactions in vivo.- Chemical Genetic Analysis of Protein Kinase Function in Plants.- Modified Metal Oxide Affinity Enrichment Combined with 2D-PAGE and Analysis of Phosphoproteomes.- Phosphoproteomics Using iTRAQ

Recenzii

From the reviews:
“This book represents a compendium of practical protocols to experimentally address different aspects of plant kinase function and regulation. It is intended to provide a benchtop manual for newcomers to the field but also to present experimental alternatives for the more specialized and advanced user. … this book compiles a wealth of information on how to study plant kinases and provides a useful set of instructions for a wide range of complementary techniques in this field.” (Jörg Kudla, Journal of Plant Physiology, Vol. 169 (15), 2012)

Textul de pe ultima copertă

Modification of target protein properties by reversible phosphorylation events has been found to be one of the most prominent cellular control processes in all organisms. Recent advances in the areas of molecular biology and biochemistry are presenting new possibilities for reaching an unprecedented depth and a proteome-wide understanding of phosphorylation processes in plants as well as in other species. The major goal of Plant Kinases: Methods and Protocols is to provide the experimentalist with a detailed account of the practical steps necessary for successfully carrying out each protocol in his or her own laboratory. Plant protein kinases specifically addressed in this volume are members of the plant MAP kinase cascade, cyclin- and Calcium-dependent protein kinases, and plant sensor and receptor kinases. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
 
Authoritative and accessible, Plant Kinases: Methods and Protocols will prove a useful laboratory companion to  both novice and seasoned researchers by facilitating the practical work that will lead them to new and exciting insights in this dynamic field.

Caracteristici

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts Includes supplementary material: sn.pub/extras