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Recombinant and In Vitro RNA Synthesis: Methods and Protocols de Graeme L. Conn

Recombinant and In Vitro RNA Synthesis: Methods and Protocols: Methods in Molecular Biology, cartea 941

Editat de Graeme L. Conn
en Limba Engleză Paperback – 23 aug 2016
The discovery of catalytic RNAs in the mid-1980s marked the beginning of a new era in RNA biology and an ever increasing appreciation of the diverse and critical roles played by this fascinating molecule. In Recombinant and In Vitro RNA Synthesis: Methods and Protocols, expert researchers in the field detail approaches from the inception of a new RNA project to the final sample ready for analysis. These experimental protocols combine established work-horse approaches with some of the latest innovative methods for RNA synthesis and purification. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
 
Authoritative and practical, Recombinant and In Vitro RNA Synthesis: Methods and Protocols aids scientists in continuing to study the fascinating world of RNA.
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Specificații

ISBN-13: 9781493958306
ISBN-10: 1493958305
Pagini: 274
Ilustrații: XIV, 274 p.
Dimensiuni: 178 x 254 mm
Greutate: 0.51 kg
Ediția:Softcover reprint of the original 1st ed. 2012
Editura: Humana Press Inc.
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:Totowa, NJ, United States

Cuprins

Purification of RNA Expressed in vivo Inserted in a tRNA Scaffold.-Selective RNase H Cleavage of Target RNAs  from a tRNA Scaffold.- Preparation of Long Templates for RNA in vitro Transcription by Recursive PCR.- General Protocols for Preparation of Plasmid DNA Template, RNA in vitro Transcription and RNA Purification by Denaturing PAGE.- Preparation of Short RNA by in vitro Transcription.- Native RNA Purification by Gel Filtration Chromatography.- Cis-acting Ribozymes for the Production of RNA in vitro Transcripts with Defined 5’ and 3’ Ends.- Trans-acting Antigenomic HDV Ribozyme for Production of in vitro Transcripts with Homogenous 3' Ends.- Rapid Preparation of RNA Samples using DNA-affinity Chromatography and DNAzyme Methods.-Preparation of lN-GST Fusion Protein for Affinity Immobilization of RNA.- Affinity Purification of RNA using an ARiBo Tag.- Plasmid Template Design and in vitro Transcription of Short RNAs within a ‘Structure Cassette’ for Structure Probing Experiments.- In Vitro Transcription of Modified RNAs.- End-Labeling Oligonucleotides with Chemical Tags After Synthesis.- High-Purity Enzymatic Synthesis of  Site-Specifically Modified tRNA.- Se-derivatized RNAs for X-ray Crystallography.- Biosynthetic Preparation of 13C/15N-labelled rNTPs for High-resolution NMR Studies of RNAs.- Preparative Separation of Ribonucleoside Monophosphates by Ion-Pair Reverse-Phase HPLC.- Splint Ligation of RNA with T4 DNA Ligase.

Textul de pe ultima copertă

The discovery of catalytic RNAs in the mid-1980s marked the beginning of a new era in RNA biology and an ever increasing appreciation of the diverse and critical roles played by this fascinating molecule. In Recombinant and In Vitro RNA Synthesis: Methods and Protocols, expert researchers in the field detail approaches from the inception of a new RNA project to the final sample ready for analysis. These experimental protocols combine established work-horse approaches with some of the latest innovative methods for RNA synthesis and purification. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
 
Authoritative and practical, Recombinant and In Vitro RNA Synthesis: Methods and Protocols aids scientists in continuing to study the fascinating world of RNA.

Caracteristici

Details a collection of protocols that guide the reader from the start of a new RNA project to the final sample Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts Includes supplementary material: sn.pub/extras Includes supplementary material: sn.pub/extras