Yeast Surface Display (Methods in Molecular Biology) de Michael W. Traxlmayr

Yeast Surface Display: Methods in Molecular Biology, cartea 2491

Michael W. Traxlmayr

en Limba Engleză Paperback – 29 apr 2023

This detailed volume explores a wide variety of applications of yeast surface display, an extensively used protein engineering technology. Beginning with detailed protocols for the construction and efficient selection/screening of yeast surface display libraries, as well as for the analysis of individual yeast-displayed protein variants, the book continues with protocols describing the selection of yeast surface display libraries for binding to mammalian cells or to extracellular matrix as well as protocols for a broad spectrum of specialized yeast surface display applications, demonstrating the versatility of this display platform. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible methodologies, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Yeast Surface Display serves as a comprehensive resource that enables the implementation of this powerful and versatile technique in virtually any molecular biology laboratory, even in the absence of any prior yeast surface display experience.

Citește tot Restrânge

Toate formatele și edițiile

	Preț	Express
Paperback (1)	1206^.06 lei 43-57 zile
Springer Us – 29 apr 2023	1206^.06 lei 43-57 zile
Hardback (1)	1636^.81 lei 43-57 zile
Springer Us – 29 apr 2022	1636^.81 lei 43-57 zile

Din seria Methods in Molecular Biology

70%

Preț: 260^.96 lei
23%

Preț: 598^.55 lei
19%

Preț: 577^.71 lei
Preț: 496^.78 lei
20%

Preț: 882^.93 lei
Preț: 252^.04 lei
5%

Preț: 708^.73 lei
5%

Preț: 710^.49 lei
5%

Preț: 720^.09 lei
5%

Preț: 725^.75 lei
15%

Preț: 644^.46 lei
18%

Preț: 995^.90 lei
5%

Preț: 713^.54 lei
18%

Preț: 887^.95 lei
15%

Preț: 645^.58 lei
15%

Preț: 635^.39 lei
18%

Preț: 1374^.06 lei
5%

Preț: 721^.34 lei
20%

Preț: 821^.63 lei
18%

Preț: 944^.40 lei
15%

Preț: 641^.58 lei
5%

Preț: 717^.27 lei
18%

Preț: 956^.66 lei
5%

Preț: 712^.29 lei
Preț: 392^.57 lei
5%

Preț: 724^.90 lei
18%

Preț: 935^.03 lei
23%

Preț: 860^.19 lei
15%

Preț: 633^.95 lei
5%

Preț: 1025^.21 lei
Preț: 792^.15 lei
Preț: 423^.61 lei
5%

Preț: 425^.90 lei
Preț: 592^.19 lei
5%

Preț: 345^.62 lei
5%

Preț: 372^.70 lei
19%

Preț: 491^.88 lei
5%

Preț: 1038^.82 lei
18%

Preț: 2061^.29 lei
5%

Preț: 1261^.92 lei
Preț: 789^.92 lei
5%

Preț: 1339^.07 lei
18%

Preț: 1350^.31 lei
5%

Preț: 752^.65 lei
5%

Preț: 374^.88 lei
18%

Preț: 1355^.52 lei
18%

Preț: 1097^.22 lei
18%

Preț: 1367^.78 lei
18%

Preț: 1092^.61 lei
18%

Preț: 938^.55 lei

Cuprins

Yeast Surface Display: New Opportunities for a Time-Tested Protein Engineering System.- Yeast Surface Display for Protein Engineering: Library Generation, Screening, and Affinity Maturation.- Site-Wise Diversification of Combinatorial Libraries Using Insights from Structure-Guided Stability Calculations.- Ancestral Sequence Reconstruction and Alternate Amino Acid States Guide Protein Library Design for Directed Evolution.- Machine Learning-Driven Protein Library Design: A Path Toward Smarter Libraries.- Kinetic Competition Screening of Yeast-Displayed Libraries for the Generation of High Affinity Binders.- Engineering Proteins by Combining Deep Mutational Scanning and Yeast Display.- Engineering Binders with Exceptional Selectivity.- Affinity and Stability Analysis of Yeast Displayed Proteins.- Antibody Library Screening Using Yeast Biopanning and Fluorescence-Activated Cell Sorting.- A Hybrid Adherent/Suspension Cell-Based Selection Strategy for Discovery of Antibodies Targeting Membrane Proteins.- Ligand Selection by Combination of Recombinant and Cell Panning Selection Techniques.- Identification of Brain ECM Binding Variable Lymphocyte Receptors Using Yeast Surface Display.- Guidelines, Strategies, and Principles for the Directed Evolution of Cross-Reactive Antibodies Using Yeast Surface Display Technology.- Yeast Display for the Identification of Peptide-MHC Ligands of Immune Receptors.- Yeast Display Guided Selection of pH Dependent Binders.- Yeast Mating as a Tool for Highly Effective Discovery and Engineering of Antibodies via Display Methodologies.- Humanization of Chicken-Derived Antibodies by Yeast Surface Display.- Engineering Tissue Inhibitors of Metalloproteinases Using Yeast Surface Display.- Discovery of Cyclic Peptide Binders from Chemically Constrained Yeast Display Libraries.- Generation of Thermally Stable Affinity Pairs for Sensitive, Specific Immunoassays.- Isolating Anti-Amyloid Antibodies from Yeast-Displayed Libraries.- EngineeringProteins Containing Noncanonical Amino Acids on the Yeast Surface.- Construction of Yeast Display Libraries for Selection of Antigen-Binding Variants of Large Extracellular Loop of CD81, a Major Surface Marker Protein of Extracellular Vesicles.- Isolating and Engineering Fluorescence-Activating Proteins Using Yeast Surface Display.- Simultaneous Display of Multiple Kinds of Enzymes on the Yeast Cell Surface for Multistep Reactions.

Textul de pe ultima copertă

Yeast Surface Display: Methods in Molecular Biology, cartea 2491

Toate formatele și edițiile

Din seria Methods in Molecular Biology

Preț: 1206^.06 lei

Carte tipărită la comandă

Specificații

Cuprins

Textul de pe ultima copertă

Caracteristici

Papetărie, jocuri, reviste

Yeast Surface Display: Methods in Molecular Biology, cartea 2491

Toate formatele și edițiile

Preț: 1206.06 lei

Specificații

V-ar putea interesa

Cuprins

Textul de pe ultima copertă

Caracteristici

Preț: 1206^.06 lei