Cantitate/Preț
Produs
Total produse
Vezi coșul
mRNA Decay: Methods and Protocols de Shireen R. Lamandé

mRNA Decay: Methods and Protocols: Methods in Molecular Biology, cartea 1720

Editat de Shireen R. Lamandé
en Limba Engleză Hardback – 15 dec 2017
This detailed volume explores the latest methods that can be used to probe mRNA decay pathways and identify mRNA-binding protein targets as well as miRNA targets. Subjects include metabolic labelling and RNAseq methods for determining RNA decay rates, approaches for discovering RNA-binding protein targets, bioinformatics, miRNA targets and novel components of the miRNA-directed decay pathway, and recently developed approaches for studying nonsense-mediated mRNA decay, among other areas. Written for the highly popular Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, mRNA Decay: Methods and Protocols serves as an ideal guide for molecular biologists, geneticists, and developmental biologists with an interest in understanding how normal development and tissue homeostasis is regulated and how these processes are perturbed in inherited and acquired diseases.
Citește tot Restrânge

Toate formatele și edițiile

Toate formatele și edițiile Preț Express
Paperback (1) 70993 lei  39-44 zile
  Springer – 30 aug 2018 70993 lei  39-44 zile
Hardback (1) 85286 lei  6-8 săpt.
  Springer – 15 dec 2017 85286 lei  6-8 săpt.

Din seria Methods in Molecular Biology

Preț: 85286 lei

Preț vechi: 89775 lei
-5% Nou

Puncte Express: 1279
Preț estimativ în valută:
16324€ • 16977$ • 13679£

Carte tipărită la comandă

Livrare economică 13-27 martie

 Adaugă în coș

Wish list
Gift list
Am citit!
Adaugă în listă
Preluare comenzi: 021 569.72.76

Specificații

ISBN-13: 9781493975396
ISBN-10: 1493975390
Ilustrații: XI, 227 p. 34 illus., 24 illus. in color. With online files/update.
Dimensiuni: 178 x 254 mm
Greutate: 0.69 kg
Ediția:1st ed. 2018
Editura: Springer
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:New York, NY, United States

Cuprins

5'bromouridine IP Chase (BRIC)-Seq to Determine RNA Half Lives.- Determining mRNA Decay Rates Using RNA Approach to Equilibrium Sequencing (RATE-Seq).- Metabolic Labeling of Newly Synthesized RNA with 4sU to in Parallel Assess RNA Transcription and Decay.- Measuring mRNA Decay in Budding Yeast Using Single Molecule FISH.- PAR-CLIP for Discovering Target Sites of RNA-Binding Proteins.- Characterizing mRNA Sequence Motifs in the 3'UTR Using GFP Reporter Constructs.- iCLIP of the PIWI Protein Aubergine in Drosophila Embryos.- Integration of ENCODE RNAseq and eCLIP Data Sets.- Identifying miRNA Targets Using AgoRIP-Seq.- Integrated Analysis of miRNA and mRNA Expression Profiles to Identify miRNA Targets.- Identifying RISC Components Using Ago2 Immunoprecipitation and Mass Spectrometry.- Using Tet-Off Cells and RNAi Knockdown to Assay mRNA Decay.- Identifying Cellular Nonsense-Mediated mRNA Decay (NMD) Targets: Immunoprecipitation of Phosphorylated UPF1 Followed by RNA Sequencing(p-UPF1 RIP-Seq).- Generation of Cell Lines Stably Expressing a Fluorescent Reporter of Nonsense-Mediated mRNA Decay Activity.- Reactivation Assay to Identify Direct Targets of the Nonsense-Mediated mRNA Decay Pathway in Drosophila.- Studying Nonsense-Mediated mRNA Decay in Mammalian Cells Using a Multicolored Bioluminescence-Based Reporter System.

Textul de pe ultima copertă

This detailed volume explores the latest methods that can be used to probe mRNA decay pathways and identify mRNA-binding protein targets as well as miRNA targets. Subjects include metabolic labelling and RNAseq methods for determining RNA decay rates, approaches for discovering RNA-binding protein targets, bioinformatics, miRNA targets and novel components of the miRNA-directed decay pathway, and recently developed approaches for studying nonsense-mediated mRNA decay, among other areas. Written for the highly popular Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, mRNA Decay: Methods and Protocols serves as an ideal guide for molecular biologists, geneticists, and developmental biologists with an interest in understanding how normal development and tissue homeostasis is regulated and how these processes are perturbed in inherited and acquired diseases.

Caracteristici

Includes cutting-edge methods and protocols for the study of mRNA decay Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts